Role of vitamin D3 as a sensitizer to cryoablation in a murine prostate cancer model: Preliminary in vivo study

Masaki Kimura, Zahid Rabbani, Vladimir Mouraviev, Matvey Tsivian, Jorge Caso, Takefumi Satoh, Shiro Baba, Zeljko Vujaskovic, John M. Baust, John G. Baust, Thomas J. Polascik

Research output: Contribution to journalArticle

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Abstract

Objectives: Calcitriol has been reported to have antitumor efficacy in several cancers. In this study, we hypothesized that calcitriol may potentially function as a cryosensitizer that can enhance cryoablation, and we investigated several molecular marker changes in a murine model of prostate cancer. Methods: Murine prostate tumors (RM-9) were grown in male C57BL/6J mice subcutaneously with neoadjuvant intratumoral injection of calcitriol followed by cryoablation. The microenvironmental changes after cryoablation alone and in combination with calcitriol were analyzed in a comparative fashion using immunohistochemistry and Western blot analyses. Results: Both cryoablation and the combination group could suppress tumor growth after treatment compared with the control. At final pathologic assessment, a larger necrotic area was seen in the combination group (P = .026). Although microvessel density (CD31) and the area of hypoxia (pimonidazole) was not different between the control and combination groups, cell proliferation (Ki-67) significantly decreased in the combination treatment (P = .035). In Western blot analyses, several markers for apoptosis were expressed significantly higher with the combination treatment. Conclusions: The synergistic effect of calcitriol with cryoablation was demonstrated because of enhanced antitumor efficacy by increasing necrosis and apoptosis and reduced cell proliferation. This study suggests that calcitriol is a potentially applicable reagent as a freeze sensitizer to cryoablation.

Original languageEnglish
JournalUrology
Volume76
Issue number3
DOIs
StatePublished - Sep 1 2010

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Cryosurgery
Calcitriol
Cholecalciferol
Prostatic Neoplasms
Western Blotting
Cell Proliferation
Apoptosis
Neoplasms
Microvessels
Inbred C57BL Mouse
Prostate
Necrosis
Therapeutics
Immunohistochemistry
Control Groups
Injections
Growth

ASJC Scopus subject areas

  • Urology

Cite this

Kimura, M., Rabbani, Z., Mouraviev, V., Tsivian, M., Caso, J., Satoh, T., ... Polascik, T. J. (2010). Role of vitamin D3 as a sensitizer to cryoablation in a murine prostate cancer model: Preliminary in vivo study. Urology, 76(3). https://doi.org/10.1016/j.urology.2010.03.041

Role of vitamin D3 as a sensitizer to cryoablation in a murine prostate cancer model : Preliminary in vivo study. / Kimura, Masaki; Rabbani, Zahid; Mouraviev, Vladimir; Tsivian, Matvey; Caso, Jorge; Satoh, Takefumi; Baba, Shiro; Vujaskovic, Zeljko; Baust, John M.; Baust, John G.; Polascik, Thomas J.

In: Urology, Vol. 76, No. 3, 01.09.2010.

Research output: Contribution to journalArticle

Kimura, M, Rabbani, Z, Mouraviev, V, Tsivian, M, Caso, J, Satoh, T, Baba, S, Vujaskovic, Z, Baust, JM, Baust, JG & Polascik, TJ 2010, 'Role of vitamin D3 as a sensitizer to cryoablation in a murine prostate cancer model: Preliminary in vivo study', Urology, vol. 76, no. 3. https://doi.org/10.1016/j.urology.2010.03.041
Kimura, Masaki ; Rabbani, Zahid ; Mouraviev, Vladimir ; Tsivian, Matvey ; Caso, Jorge ; Satoh, Takefumi ; Baba, Shiro ; Vujaskovic, Zeljko ; Baust, John M. ; Baust, John G. ; Polascik, Thomas J. / Role of vitamin D3 as a sensitizer to cryoablation in a murine prostate cancer model : Preliminary in vivo study. In: Urology. 2010 ; Vol. 76, No. 3.
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