Role of twin Cys-Xaa9-Cys motif cysteines in mitochondrial import of the cytochrome c oxidase biogenesis factor Cmc1

Myriam Bourens, Deepa V. Dabir, Heather L. Tienson, Irina Sorokina, Carla M. Koehler, Antonio Barrientos

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Mia40 import pathway facilitates the import and oxidative folding of cysteine-rich protein substrates into the mitochondrial intermembrane space. Here we describe the in vitro and in organello oxidative folding of Cmc1, a twin CX9C-containing substrate, which contains an unpaired cysteine. In vitro, Cmc1 can be oxidized by the import receptor Mia40 alone when in excess or at a lower rate by only the sulfhydryl oxidase Erv1. However, physiological and efficient Cmc1 oxidation requires Erv1 and Mia40. Cmc1 forms a stable intermediate with Mia40 and is released from this interaction in the presence of Erv1. The three proteins are shown to form a ternary complex in mitochondria. Our results suggest that this mechanism facilitates efficient formation of multiple disulfides and prevents the formation of non-native disulfide bonds.

Original languageEnglish
Pages (from-to)31258-31269
Number of pages12
JournalJournal of Biological Chemistry
Volume287
Issue number37
DOIs
StatePublished - Sep 7 2012

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Electron Transport Complex IV
Disulfides
Cysteine
Mitochondria
Substrates
Proteins
Oxidation
In Vitro Techniques
sulfhydryl oxidase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Role of twin Cys-Xaa9-Cys motif cysteines in mitochondrial import of the cytochrome c oxidase biogenesis factor Cmc1. / Bourens, Myriam; Dabir, Deepa V.; Tienson, Heather L.; Sorokina, Irina; Koehler, Carla M.; Barrientos, Antonio.

In: Journal of Biological Chemistry, Vol. 287, No. 37, 07.09.2012, p. 31258-31269.

Research output: Contribution to journalArticle

Bourens, Myriam ; Dabir, Deepa V. ; Tienson, Heather L. ; Sorokina, Irina ; Koehler, Carla M. ; Barrientos, Antonio. / Role of twin Cys-Xaa9-Cys motif cysteines in mitochondrial import of the cytochrome c oxidase biogenesis factor Cmc1. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 37. pp. 31258-31269.
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