Role of tissue digestion and extensive sperm search after microdissection testicular sperm extraction

Ranjith Ramasamy, Jennifer E. Reifsnyder, Campbell Bryson, Nikica Zaninovic, Deborah Liotta, Carol Ann Cook, June Hariprashad, Dina Weiss, Queenie Neri, Gianpiero D. Palermo, Peter N. Schlegel

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: To report the chance of sperm discovery in the laboratory when sperm were not identified in the operating room (OR). Design: Clinical retrospective study. Setting: Department of urology at a tertiary university hospital. Patient(s): A total of 1,054 men with nonobstructive azoospermia who underwent microdissection testicular sperm extraction. Intervention(s): Preoperative and intraoperative parameters were analyzed relative to the chance of sperm identification using a tissue digestion protocol in the laboratory if no sperm were observed in the OR. Main Outcome Measure(s): Sperm retrieval, clinical pregnancy, and live birth rates. Result(s): Sperm were found in the OR in 52.5% of the 1,054 men. Of the 501 men for whom sperm were not identified by andrologists in the OR, sperm were found in the laboratory for an additional 35 (7%). On multivariable logistic regression analysis, the presence of germ cells intraoperatively was the only predictor of identifying sperm in the laboratory after tissue digestion. Conclusion(s): In men undergoing microdissection testicular sperm extraction, when sperm were not observed in the OR despite extensive mechanical processing, sperm were observed in the laboratory for 7% of the men. This information is valuable in counseling couples in the immediate postoperative period when no sperm were identified intraoperatively.

Original languageEnglish (US)
Pages (from-to)299-302
Number of pages4
JournalFertility and Sterility
Volume96
Issue number2
DOIs
StatePublished - Aug 2011
Externally publishedYes

Fingerprint

Microdissection
Spermatozoa
Digestion
Operating Rooms
Sperm Retrieval
Urology
Pregnancy Rate
Postoperative Period
Tertiary Care Centers
Germ Cells
Counseling

Keywords

  • Azoospermia
  • biopsy
  • FSH
  • microdissection
  • predictors
  • pregnancy
  • TESE
  • testis

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Role of tissue digestion and extensive sperm search after microdissection testicular sperm extraction. / Ramasamy, Ranjith; Reifsnyder, Jennifer E.; Bryson, Campbell; Zaninovic, Nikica; Liotta, Deborah; Cook, Carol Ann; Hariprashad, June; Weiss, Dina; Neri, Queenie; Palermo, Gianpiero D.; Schlegel, Peter N.

In: Fertility and Sterility, Vol. 96, No. 2, 08.2011, p. 299-302.

Research output: Contribution to journalArticle

Ramasamy, R, Reifsnyder, JE, Bryson, C, Zaninovic, N, Liotta, D, Cook, CA, Hariprashad, J, Weiss, D, Neri, Q, Palermo, GD & Schlegel, PN 2011, 'Role of tissue digestion and extensive sperm search after microdissection testicular sperm extraction', Fertility and Sterility, vol. 96, no. 2, pp. 299-302. https://doi.org/10.1016/j.fertnstert.2011.05.033
Ramasamy, Ranjith ; Reifsnyder, Jennifer E. ; Bryson, Campbell ; Zaninovic, Nikica ; Liotta, Deborah ; Cook, Carol Ann ; Hariprashad, June ; Weiss, Dina ; Neri, Queenie ; Palermo, Gianpiero D. ; Schlegel, Peter N. / Role of tissue digestion and extensive sperm search after microdissection testicular sperm extraction. In: Fertility and Sterility. 2011 ; Vol. 96, No. 2. pp. 299-302.
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