Role of the Yersinia pestis ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague

B. Joseph Hinnebusch, Clayton O. Jarrett, Julie A. Callison, Donald Gardner, Susan K. Buchanan, Gregory V Plano

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The ability of Yersinia pestis to forestall the mammalian innate immune response is a fundamental aspect of plague pathogenesis. In this study, we examined the effect of Ail, a 17-kDa outer membrane protein that protects Y. pestis against complement-mediated lysis, on bubonic plague pathogenesis in mice and rats. The Y. pestis ail mutant was attenuated for virulence in both rodent models. The attenuation was greater in rats than in mice, which correlates with the ability of normal rat serum, but not mouse serum, to kill ail-negative Y. pestis in vitro. Intradermal infection with the ail mutant resulted in an atypical, subacute form of bubonic plague associated with extensive recruitment of polymorphonuclear leukocytes (PMN or neutrophils) to the site of infection in the draining lymph node and the formation of large purulent abscesses that contained the bacteria. Systemic spread and mortality were greatly attenuated, however, and a productive adaptive immune response was generated after high-dose challenge, as evidenced by high serum antibody levels against Y. pestis F1 antigen. The Y. pestis Ail protein is an important bubonic plague virulence factor that inhibits the innate immune response, in particular the recruitment of a protective PMN response to the infected lymph node.

Original languageEnglish
Pages (from-to)4984-4989
Number of pages6
JournalInfection and Immunity
Volume79
Issue number12
DOIs
StatePublished - Dec 1 2011

Fingerprint

Yersinia pestis
Plague
Neutrophils
Innate Immunity
Lymph Nodes
Serum
Adaptive Immunity
Virulence Factors
Infection
Abscess
Virulence
Rodentia
Membrane Proteins
Bacteria
Mortality
Yersinia pestis Ail protein
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Role of the Yersinia pestis ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague. / Joseph Hinnebusch, B.; Jarrett, Clayton O.; Callison, Julie A.; Gardner, Donald; Buchanan, Susan K.; Plano, Gregory V.

In: Infection and Immunity, Vol. 79, No. 12, 01.12.2011, p. 4984-4989.

Research output: Contribution to journalArticle

Joseph Hinnebusch, B. ; Jarrett, Clayton O. ; Callison, Julie A. ; Gardner, Donald ; Buchanan, Susan K. ; Plano, Gregory V. / Role of the Yersinia pestis ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague. In: Infection and Immunity. 2011 ; Vol. 79, No. 12. pp. 4984-4989.
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