Role of the lens capsule on the mechanical accommodative response in a lens stretcher

Noel Marysa Ziebarth, David Borja, Esdras Arrieta Quintero, Mohamed Aly, Fabrice Manns, Isabelle Dortonne, Derek Nankivil, Rakhi Jain, Jean-Marie A Parel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

PURPOSE. To determine whether changes in elastic properties of the lens capsule ex vivo with age contribute to the forces necessary for accommodation. METHODS. Postmortem human (n = 22; age average: 41 ± 17years; range: 6-7) and cynomolgus monkey (n = 19; age average: 7.7 ± 1.8 years, range: 4.2-10) tissues including the lens, capsule, zonules, ciliary body, and sclera were mounted in an optomechanical lens-stretching system. Starting at 0 load, the sclera was symmetrically stretched to 2 mm in 0.25-mm steps at a speed of 0.1 mm·s-1. The load and lens diameter were measured at each step. The lens contents were removed through a mini-capsulorhexis. The stretching cycles were repeated on the empty capsular bag. The forces necessary to stretch the natural lens and empty bag were quantified as a function of age and compared. RESULTS. The force needed to stretch the empty lens capsule was independent of age (human, 2.6-34.9 g/mm [25.2-342.7 mN/mm]; monkey, 8.2-21.3 g/mm [80.3-208.6 mN/mm]). The ratio of the force necessary to stretch the empty lens capsule to the force necessary to stretch the natural lens decreased with age in the human and monkey lenses (P = 0.003, P = 0.72, respectively). CONCLUSIONS. The mechanical properties of the empty lens capsule assessed ex vivo in a lens stretcher remain constant with age, suggesting that the changes in elasticity of the lens capsule do not play a significant role in presbyopia. In young eyes, the lens capsule determines the force necessary to stretch the whole lens. The age-related increase in force needed to stretch the lens is due to changes in the lens contents.

Original languageEnglish
Pages (from-to)4490-4496
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume49
Issue number10
DOIs
StatePublished - Oct 1 2008

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Lenses
Capsules
Sclera
Haplorhini
Presbyopia
Capsulorhexis
Crystalline Lens
Ciliary Body
Macaca fascicularis
Elasticity

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

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Role of the lens capsule on the mechanical accommodative response in a lens stretcher. / Ziebarth, Noel Marysa; Borja, David; Arrieta Quintero, Esdras; Aly, Mohamed; Manns, Fabrice; Dortonne, Isabelle; Nankivil, Derek; Jain, Rakhi; Parel, Jean-Marie A.

In: Investigative Ophthalmology and Visual Science, Vol. 49, No. 10, 01.10.2008, p. 4490-4496.

Research output: Contribution to journalArticle

Ziebarth, Noel Marysa ; Borja, David ; Arrieta Quintero, Esdras ; Aly, Mohamed ; Manns, Fabrice ; Dortonne, Isabelle ; Nankivil, Derek ; Jain, Rakhi ; Parel, Jean-Marie A. / Role of the lens capsule on the mechanical accommodative response in a lens stretcher. In: Investigative Ophthalmology and Visual Science. 2008 ; Vol. 49, No. 10. pp. 4490-4496.
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abstract = "PURPOSE. To determine whether changes in elastic properties of the lens capsule ex vivo with age contribute to the forces necessary for accommodation. METHODS. Postmortem human (n = 22; age average: 41 ± 17years; range: 6-7) and cynomolgus monkey (n = 19; age average: 7.7 ± 1.8 years, range: 4.2-10) tissues including the lens, capsule, zonules, ciliary body, and sclera were mounted in an optomechanical lens-stretching system. Starting at 0 load, the sclera was symmetrically stretched to 2 mm in 0.25-mm steps at a speed of 0.1 mm·s-1. The load and lens diameter were measured at each step. The lens contents were removed through a mini-capsulorhexis. The stretching cycles were repeated on the empty capsular bag. The forces necessary to stretch the natural lens and empty bag were quantified as a function of age and compared. RESULTS. The force needed to stretch the empty lens capsule was independent of age (human, 2.6-34.9 g/mm [25.2-342.7 mN/mm]; monkey, 8.2-21.3 g/mm [80.3-208.6 mN/mm]). The ratio of the force necessary to stretch the empty lens capsule to the force necessary to stretch the natural lens decreased with age in the human and monkey lenses (P = 0.003, P = 0.72, respectively). CONCLUSIONS. The mechanical properties of the empty lens capsule assessed ex vivo in a lens stretcher remain constant with age, suggesting that the changes in elasticity of the lens capsule do not play a significant role in presbyopia. In young eyes, the lens capsule determines the force necessary to stretch the whole lens. The age-related increase in force needed to stretch the lens is due to changes in the lens contents.",
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AU - Ziebarth, Noel Marysa

AU - Borja, David

AU - Arrieta Quintero, Esdras

AU - Aly, Mohamed

AU - Manns, Fabrice

AU - Dortonne, Isabelle

AU - Nankivil, Derek

AU - Jain, Rakhi

AU - Parel, Jean-Marie A

PY - 2008/10/1

Y1 - 2008/10/1

N2 - PURPOSE. To determine whether changes in elastic properties of the lens capsule ex vivo with age contribute to the forces necessary for accommodation. METHODS. Postmortem human (n = 22; age average: 41 ± 17years; range: 6-7) and cynomolgus monkey (n = 19; age average: 7.7 ± 1.8 years, range: 4.2-10) tissues including the lens, capsule, zonules, ciliary body, and sclera were mounted in an optomechanical lens-stretching system. Starting at 0 load, the sclera was symmetrically stretched to 2 mm in 0.25-mm steps at a speed of 0.1 mm·s-1. The load and lens diameter were measured at each step. The lens contents were removed through a mini-capsulorhexis. The stretching cycles were repeated on the empty capsular bag. The forces necessary to stretch the natural lens and empty bag were quantified as a function of age and compared. RESULTS. The force needed to stretch the empty lens capsule was independent of age (human, 2.6-34.9 g/mm [25.2-342.7 mN/mm]; monkey, 8.2-21.3 g/mm [80.3-208.6 mN/mm]). The ratio of the force necessary to stretch the empty lens capsule to the force necessary to stretch the natural lens decreased with age in the human and monkey lenses (P = 0.003, P = 0.72, respectively). CONCLUSIONS. The mechanical properties of the empty lens capsule assessed ex vivo in a lens stretcher remain constant with age, suggesting that the changes in elasticity of the lens capsule do not play a significant role in presbyopia. In young eyes, the lens capsule determines the force necessary to stretch the whole lens. The age-related increase in force needed to stretch the lens is due to changes in the lens contents.

AB - PURPOSE. To determine whether changes in elastic properties of the lens capsule ex vivo with age contribute to the forces necessary for accommodation. METHODS. Postmortem human (n = 22; age average: 41 ± 17years; range: 6-7) and cynomolgus monkey (n = 19; age average: 7.7 ± 1.8 years, range: 4.2-10) tissues including the lens, capsule, zonules, ciliary body, and sclera were mounted in an optomechanical lens-stretching system. Starting at 0 load, the sclera was symmetrically stretched to 2 mm in 0.25-mm steps at a speed of 0.1 mm·s-1. The load and lens diameter were measured at each step. The lens contents were removed through a mini-capsulorhexis. The stretching cycles were repeated on the empty capsular bag. The forces necessary to stretch the natural lens and empty bag were quantified as a function of age and compared. RESULTS. The force needed to stretch the empty lens capsule was independent of age (human, 2.6-34.9 g/mm [25.2-342.7 mN/mm]; monkey, 8.2-21.3 g/mm [80.3-208.6 mN/mm]). The ratio of the force necessary to stretch the empty lens capsule to the force necessary to stretch the natural lens decreased with age in the human and monkey lenses (P = 0.003, P = 0.72, respectively). CONCLUSIONS. The mechanical properties of the empty lens capsule assessed ex vivo in a lens stretcher remain constant with age, suggesting that the changes in elasticity of the lens capsule do not play a significant role in presbyopia. In young eyes, the lens capsule determines the force necessary to stretch the whole lens. The age-related increase in force needed to stretch the lens is due to changes in the lens contents.

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