Role of the initial state distribution on first-order deactivation of microheterogeneous enzyme samples

Arum Malhotra, Ajit Sadana

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The presence of microheterogeneity in enzymes is reviewed and its role in enzyme deactivation kinetics is examined. It is shown that discrete state and continuous distribution analysis of microheterogeneity are very similar. Continuous distribution of the thermal deactivation activation energy is assumed. Using a simple first-order deactivation model, the role of the initial shape and spread of this distribution is examined. Symmetrical distributions are seen to have very similar effects on deactivation kinetics. For asymmetrical distributions, skew of the distribution is shown to be an important parameter. The analysis shows that very detailed deactivation data is necessary to distinguish between different distributions of deactivation activation energies in enzymes.

Original languageEnglish (US)
Pages (from-to)143-162
Number of pages20
JournalJournal of theoretical biology
Volume145
Issue number2
DOIs
StatePublished - Jul 24 1990
Externally publishedYes

ASJC Scopus subject areas

  • Statistics and Probability
  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

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