Role of serotonin on cocaine-mediated effects on prodynorphin gene expression in the rat brain

Manuela Di Benedetto, Claudio D'Addario, Stephanie Collins, Sari E Izenwasser, Sanzio Candeletti, Patrizia Romualdi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of the selective serotonin uptake inhibitor fluoxetine was examined on prodynorphin gene expression. Fluoxetine or vehicle was infused continuously for 7 d via osmotic minipumps into male rats. Northern blot analysis showed significant increases in prodynorphin gene expression in the hypothalamus (171% of controls) and significant decreases in the caudate putamen and nucleus accumbens (62% and 70% of controls, respectively). There were no significant changes in the hippocampus. Thus, chronic inhibition of serotonin uptake can regulate prodynorphin gene expression in the hypothalamus, caudate putamen, and nucleus accumbens. Fluoxetine effects were also evaluated in rats treated with p-chloroamphetamine (PCA), a neurotoxin that depletes serotonin. Because we previously reported that continuous infusion of cocaine for 7 d (which inhibits dopamine, serotonin, and norepinephrine uptake), or GBR12909 (a selective dopamine uptake inhibitor), produced significant decreases in the hypothalamus and cocaine also produced a significant increase in prodynorphin gene expression in caudate putamen, regulation of prodynorphin gene expression by fluoxetine is suggested to be different from that by cocaine. Because neither a selective dopamine uptake inhibitor nor a selective serotonin uptake inhibitor produced the same effect as cocaine in the caudate putamen, this effect is likely regulated by the inhibition of norepinephrine uptake, by a combination of effects on two or three neurotransmitter transporters, or by a mechanism unrelated to transporter inhibition.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalJournal of Molecular Neuroscience
Volume22
Issue number3
DOIs
StatePublished - Mar 1 2004

Fingerprint

Cocaine
Gene expression
Fluoxetine
Putamen
Rats
Brain
Serotonin
Gene Expression
Dopamine Uptake Inhibitors
Hypothalamus
Caudate Nucleus
Nucleus Accumbens
Serotonin Uptake Inhibitors
Norepinephrine
Neurotransmitter Transport Proteins
p-Chloroamphetamine
Neurotoxins
Gene Expression Regulation
Northern Blotting
Hippocampus

Keywords

  • Fluoxetine
  • Opioid
  • P-chloroamphetamine
  • Prodynorphin gene expression
  • Rat
  • Serotonin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry
  • Genetics

Cite this

Role of serotonin on cocaine-mediated effects on prodynorphin gene expression in the rat brain. / Di Benedetto, Manuela; D'Addario, Claudio; Collins, Stephanie; Izenwasser, Sari E; Candeletti, Sanzio; Romualdi, Patrizia.

In: Journal of Molecular Neuroscience, Vol. 22, No. 3, 01.03.2004, p. 213-222.

Research output: Contribution to journalArticle

Di Benedetto, Manuela ; D'Addario, Claudio ; Collins, Stephanie ; Izenwasser, Sari E ; Candeletti, Sanzio ; Romualdi, Patrizia. / Role of serotonin on cocaine-mediated effects on prodynorphin gene expression in the rat brain. In: Journal of Molecular Neuroscience. 2004 ; Vol. 22, No. 3. pp. 213-222.
@article{e287e954b07a42c1bca0dedf846ac8b2,
title = "Role of serotonin on cocaine-mediated effects on prodynorphin gene expression in the rat brain",
abstract = "The effect of the selective serotonin uptake inhibitor fluoxetine was examined on prodynorphin gene expression. Fluoxetine or vehicle was infused continuously for 7 d via osmotic minipumps into male rats. Northern blot analysis showed significant increases in prodynorphin gene expression in the hypothalamus (171{\%} of controls) and significant decreases in the caudate putamen and nucleus accumbens (62{\%} and 70{\%} of controls, respectively). There were no significant changes in the hippocampus. Thus, chronic inhibition of serotonin uptake can regulate prodynorphin gene expression in the hypothalamus, caudate putamen, and nucleus accumbens. Fluoxetine effects were also evaluated in rats treated with p-chloroamphetamine (PCA), a neurotoxin that depletes serotonin. Because we previously reported that continuous infusion of cocaine for 7 d (which inhibits dopamine, serotonin, and norepinephrine uptake), or GBR12909 (a selective dopamine uptake inhibitor), produced significant decreases in the hypothalamus and cocaine also produced a significant increase in prodynorphin gene expression in caudate putamen, regulation of prodynorphin gene expression by fluoxetine is suggested to be different from that by cocaine. Because neither a selective dopamine uptake inhibitor nor a selective serotonin uptake inhibitor produced the same effect as cocaine in the caudate putamen, this effect is likely regulated by the inhibition of norepinephrine uptake, by a combination of effects on two or three neurotransmitter transporters, or by a mechanism unrelated to transporter inhibition.",
keywords = "Fluoxetine, Opioid, P-chloroamphetamine, Prodynorphin gene expression, Rat, Serotonin",
author = "{Di Benedetto}, Manuela and Claudio D'Addario and Stephanie Collins and Izenwasser, {Sari E} and Sanzio Candeletti and Patrizia Romualdi",
year = "2004",
month = "3",
day = "1",
doi = "10.1385/JMN:22:3:213",
language = "English",
volume = "22",
pages = "213--222",
journal = "Journal of Molecular Neuroscience",
issn = "0895-8696",
publisher = "Humana Press",
number = "3",

}

TY - JOUR

T1 - Role of serotonin on cocaine-mediated effects on prodynorphin gene expression in the rat brain

AU - Di Benedetto, Manuela

AU - D'Addario, Claudio

AU - Collins, Stephanie

AU - Izenwasser, Sari E

AU - Candeletti, Sanzio

AU - Romualdi, Patrizia

PY - 2004/3/1

Y1 - 2004/3/1

N2 - The effect of the selective serotonin uptake inhibitor fluoxetine was examined on prodynorphin gene expression. Fluoxetine or vehicle was infused continuously for 7 d via osmotic minipumps into male rats. Northern blot analysis showed significant increases in prodynorphin gene expression in the hypothalamus (171% of controls) and significant decreases in the caudate putamen and nucleus accumbens (62% and 70% of controls, respectively). There were no significant changes in the hippocampus. Thus, chronic inhibition of serotonin uptake can regulate prodynorphin gene expression in the hypothalamus, caudate putamen, and nucleus accumbens. Fluoxetine effects were also evaluated in rats treated with p-chloroamphetamine (PCA), a neurotoxin that depletes serotonin. Because we previously reported that continuous infusion of cocaine for 7 d (which inhibits dopamine, serotonin, and norepinephrine uptake), or GBR12909 (a selective dopamine uptake inhibitor), produced significant decreases in the hypothalamus and cocaine also produced a significant increase in prodynorphin gene expression in caudate putamen, regulation of prodynorphin gene expression by fluoxetine is suggested to be different from that by cocaine. Because neither a selective dopamine uptake inhibitor nor a selective serotonin uptake inhibitor produced the same effect as cocaine in the caudate putamen, this effect is likely regulated by the inhibition of norepinephrine uptake, by a combination of effects on two or three neurotransmitter transporters, or by a mechanism unrelated to transporter inhibition.

AB - The effect of the selective serotonin uptake inhibitor fluoxetine was examined on prodynorphin gene expression. Fluoxetine or vehicle was infused continuously for 7 d via osmotic minipumps into male rats. Northern blot analysis showed significant increases in prodynorphin gene expression in the hypothalamus (171% of controls) and significant decreases in the caudate putamen and nucleus accumbens (62% and 70% of controls, respectively). There were no significant changes in the hippocampus. Thus, chronic inhibition of serotonin uptake can regulate prodynorphin gene expression in the hypothalamus, caudate putamen, and nucleus accumbens. Fluoxetine effects were also evaluated in rats treated with p-chloroamphetamine (PCA), a neurotoxin that depletes serotonin. Because we previously reported that continuous infusion of cocaine for 7 d (which inhibits dopamine, serotonin, and norepinephrine uptake), or GBR12909 (a selective dopamine uptake inhibitor), produced significant decreases in the hypothalamus and cocaine also produced a significant increase in prodynorphin gene expression in caudate putamen, regulation of prodynorphin gene expression by fluoxetine is suggested to be different from that by cocaine. Because neither a selective dopamine uptake inhibitor nor a selective serotonin uptake inhibitor produced the same effect as cocaine in the caudate putamen, this effect is likely regulated by the inhibition of norepinephrine uptake, by a combination of effects on two or three neurotransmitter transporters, or by a mechanism unrelated to transporter inhibition.

KW - Fluoxetine

KW - Opioid

KW - P-chloroamphetamine

KW - Prodynorphin gene expression

KW - Rat

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=2942512822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942512822&partnerID=8YFLogxK

U2 - 10.1385/JMN:22:3:213

DO - 10.1385/JMN:22:3:213

M3 - Article

C2 - 14997015

AN - SCOPUS:2942512822

VL - 22

SP - 213

EP - 222

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

SN - 0895-8696

IS - 3

ER -