Role of scarf and its binding target proteins in epidermal calcium homeostasis

Joonsung Hwang, Alexandr Kalinin, Meeyul Hwang, D. Eric Anderson, Jung Kim Min, Olivera Stojadinovic, Marjana Tomic-Canic, Hun Lee Seung, Maria I. Morasso

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The novel Ca2+-binding protein, Scarf (skin calmodulin-related factor) belongs to the calmodulin-like protein family and is expressed in the differentiated layers of the epidermis. To determine the roles of Scarf during stratification, we set out to identify the binding target proteins by affinity chromatography and subsequent analysis by mass spectrometry. Several binding factors, including 14-3-3s, annexins, calreticulin, ERp72 (endoplasmic reticulum protein 72), and nucleolin, were identified, and their interactions with Scarf were corroborated by co-immunoprecipitation and co-localization analyses. To further understand the functions of Scarf in epidermis in vivo, we altered the epidermal Ca2+ gradient by acute barrier disruption. The change in the expression levels of Scarf and its binding target proteins were determined by immunohistochemistry and Western blot analysis. The expression of Scarf, annexins, calreticulin, and ERp72 were up-regulated by Ca2+ gradient disruption, whereas the expression of 14-3-3s and nucleolin was reduced. Because annexins, calreticulin, and ERp72 have been implicated in Ca 2+-induced cellular trafficking, including the secretion of lamellar bodies and Ca2+ homeostasis, we propose that the interaction of Scarf with these proteins might be crucial in the process of barrier restoration. On the other hand, down-regulation of 14-3-3s and nucleolin is potentially involved in the process of keratinocyte differentiation and growth inhibition. The calcium-dependent localization and up-regulation of Scarf and its binding target proteins were studied in mouse keratinocytes treated with ionomycin and during the wound-healing process. We found increased expression and nuclear presence of Scarf in the epidermis of the wound edge 4 and 7 days post-wounding, entailing the role of Scarf in barrier restoration. Our results suggest that Scarf plays a critical role as a Ca2+ sensor, potentially regulating the function of its binding target proteins in a Ca2+-dependent manner in the process of restoration of epidermal Ca2+ gradient as well as during epidermal barrier formation.

Original languageEnglish
Pages (from-to)18645-18653
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number25
DOIs
StatePublished - Jun 22 2007
Externally publishedYes

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Calmodulin
Carrier Proteins
Homeostasis
Skin
Calcium
Proteins
Calreticulin
Annexins
Epidermis
Endoplasmic Reticulum
Restoration
Keratinocytes
Calmodulin-Binding Proteins
Ionomycin
Affinity chromatography
Affinity Chromatography
Immunoprecipitation
Wound Healing
Mass Spectrometry
Mass spectrometry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hwang, J., Kalinin, A., Hwang, M., Anderson, D. E., Min, J. K., Stojadinovic, O., ... Morasso, M. I. (2007). Role of scarf and its binding target proteins in epidermal calcium homeostasis. Journal of Biological Chemistry, 282(25), 18645-18653. https://doi.org/10.1074/jbc.M702035200

Role of scarf and its binding target proteins in epidermal calcium homeostasis. / Hwang, Joonsung; Kalinin, Alexandr; Hwang, Meeyul; Anderson, D. Eric; Min, Jung Kim; Stojadinovic, Olivera; Tomic-Canic, Marjana; Seung, Hun Lee; Morasso, Maria I.

In: Journal of Biological Chemistry, Vol. 282, No. 25, 22.06.2007, p. 18645-18653.

Research output: Contribution to journalArticle

Hwang, J, Kalinin, A, Hwang, M, Anderson, DE, Min, JK, Stojadinovic, O, Tomic-Canic, M, Seung, HL & Morasso, MI 2007, 'Role of scarf and its binding target proteins in epidermal calcium homeostasis', Journal of Biological Chemistry, vol. 282, no. 25, pp. 18645-18653. https://doi.org/10.1074/jbc.M702035200
Hwang J, Kalinin A, Hwang M, Anderson DE, Min JK, Stojadinovic O et al. Role of scarf and its binding target proteins in epidermal calcium homeostasis. Journal of Biological Chemistry. 2007 Jun 22;282(25):18645-18653. https://doi.org/10.1074/jbc.M702035200
Hwang, Joonsung ; Kalinin, Alexandr ; Hwang, Meeyul ; Anderson, D. Eric ; Min, Jung Kim ; Stojadinovic, Olivera ; Tomic-Canic, Marjana ; Seung, Hun Lee ; Morasso, Maria I. / Role of scarf and its binding target proteins in epidermal calcium homeostasis. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 25. pp. 18645-18653.
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AU - Tomic-Canic, Marjana

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AU - Morasso, Maria I.

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