Role of reactive oxygen species in contraction-mediated glucose transport in mouse skeletal muscle

Marie E. Sandström, Shi Jin Zhang, Joseph Bruton, José P. Silva, Michael B. Reid, Håkan Westerblad, Abram Katz

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Exercise increases glucose transport into skeletal muscle via a pathway that is poorly understood. We investigated the role of endogenously produced reactive oxygen species (ROS) in contraction-mediated glucose transport. Repeated contractions increased 2-deoxyglucose (2-DG) uptake roughly threefold in isolated, mouse extensor digitorum longus (fast-twitch) muscle. N-Acetylcysteine (NAC), a non-specific antioxidant, inhibited contraction-mediated 2-DG uptake by ∼50% (P < 0.05 versus control values), but did not significantly affect basal 2-DG uptake or the uptake induced by insulin, hypoxia or 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR, which mimics AMP-mediated activation of AMP-activated protein kinase, AMPK). Ebselen, a glutathione peroxidase mimetic, also inhibited contraction-mediated 2-DG uptake (by almost 60%, P < 0.001 versus control values). Muscles from mice overexpressing Mn2+- dependent superoxide dismutase, which catalyses H2 O2 production from superoxide anions, exhibited a ∼25% higher rate of contraction-mediated 2-DG uptake versus muscles from wild-type control mice (P < 0.05). Exogenous H2 O2 induced oxidative stress, as judged by an increase in the [GSSG]/[GSH + GSSG] (reduced glutathione + oxidized glutathione) ratio to 2.5 times control values, and this increase was substantially blocked by NAC. Similarly, NAC significantly attenuated contraction-mediated oxidative stress as judged by measurements of glutathione status and the intracellular ROS level with the fluorescent indicator 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein (P < 0.05). Finally, contraction increased AMPK activity and phosphorylation ∼10-fold, and NAC blocked ∼50% of these changes. These data indicate that endogenously produced ROS, possibly H2O2 or its derivatives, play an important role in contraction-mediated activation of glucose transport in fast-twitch muscle.

Original languageEnglish
Pages (from-to)251-262
Number of pages12
JournalJournal of Physiology
Volume575
Issue number1
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

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Deoxyglucose
Acetylcysteine
Reactive Oxygen Species
Skeletal Muscle
AMP-Activated Protein Kinases
Glucose
Muscles
Glutathione Disulfide
Glutathione
Oxidative Stress
Adenosine Monophosphate
Glutathione Peroxidase
Superoxides
Superoxide Dismutase
Antioxidants
Phosphorylation
Insulin

ASJC Scopus subject areas

  • Physiology

Cite this

Sandström, M. E., Zhang, S. J., Bruton, J., Silva, J. P., Reid, M. B., Westerblad, H., & Katz, A. (2006). Role of reactive oxygen species in contraction-mediated glucose transport in mouse skeletal muscle. Journal of Physiology, 575(1), 251-262. https://doi.org/10.1113/jphysiol.2006.110601

Role of reactive oxygen species in contraction-mediated glucose transport in mouse skeletal muscle. / Sandström, Marie E.; Zhang, Shi Jin; Bruton, Joseph; Silva, José P.; Reid, Michael B.; Westerblad, Håkan; Katz, Abram.

In: Journal of Physiology, Vol. 575, No. 1, 01.08.2006, p. 251-262.

Research output: Contribution to journalArticle

Sandström, ME, Zhang, SJ, Bruton, J, Silva, JP, Reid, MB, Westerblad, H & Katz, A 2006, 'Role of reactive oxygen species in contraction-mediated glucose transport in mouse skeletal muscle', Journal of Physiology, vol. 575, no. 1, pp. 251-262. https://doi.org/10.1113/jphysiol.2006.110601
Sandström, Marie E. ; Zhang, Shi Jin ; Bruton, Joseph ; Silva, José P. ; Reid, Michael B. ; Westerblad, Håkan ; Katz, Abram. / Role of reactive oxygen species in contraction-mediated glucose transport in mouse skeletal muscle. In: Journal of Physiology. 2006 ; Vol. 575, No. 1. pp. 251-262.
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abstract = "Exercise increases glucose transport into skeletal muscle via a pathway that is poorly understood. We investigated the role of endogenously produced reactive oxygen species (ROS) in contraction-mediated glucose transport. Repeated contractions increased 2-deoxyglucose (2-DG) uptake roughly threefold in isolated, mouse extensor digitorum longus (fast-twitch) muscle. N-Acetylcysteine (NAC), a non-specific antioxidant, inhibited contraction-mediated 2-DG uptake by ∼50{\%} (P < 0.05 versus control values), but did not significantly affect basal 2-DG uptake or the uptake induced by insulin, hypoxia or 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR, which mimics AMP-mediated activation of AMP-activated protein kinase, AMPK). Ebselen, a glutathione peroxidase mimetic, also inhibited contraction-mediated 2-DG uptake (by almost 60{\%}, P < 0.001 versus control values). Muscles from mice overexpressing Mn2+- dependent superoxide dismutase, which catalyses H2 O2 production from superoxide anions, exhibited a ∼25{\%} higher rate of contraction-mediated 2-DG uptake versus muscles from wild-type control mice (P < 0.05). Exogenous H2 O2 induced oxidative stress, as judged by an increase in the [GSSG]/[GSH + GSSG] (reduced glutathione + oxidized glutathione) ratio to 2.5 times control values, and this increase was substantially blocked by NAC. Similarly, NAC significantly attenuated contraction-mediated oxidative stress as judged by measurements of glutathione status and the intracellular ROS level with the fluorescent indicator 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein (P < 0.05). Finally, contraction increased AMPK activity and phosphorylation ∼10-fold, and NAC blocked ∼50{\%} of these changes. These data indicate that endogenously produced ROS, possibly H2O2 or its derivatives, play an important role in contraction-mediated activation of glucose transport in fast-twitch muscle.",
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