Role of phosphoinositide hydrolysis in astrocyte volume regulation

A. S. Bender, J. T. Neary, Michael D Norenberg

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Astrocytes exposed to hypoosmotic stress swell and subsequently reduce their size to almost their original volume, a phenomenon called regulatory volume decrease (RVD). We found that during hypoosmotic swelling there was a twofold increase in phosphatidylinositol (PI) hydrolysis. This increase was inhibited by the phospholipase C inhibitor, U-73122 (10 μM). Inhibition of PI hydrolysis resulted in blockage of RVD. We also examined whether agents that stimulate PI hydrolysis would enhance RVD. These agents significantly accelerated RVD. The rank order of potency was endothelin (20 nM) ≥ norepinephrine (100 μM) > endothelin-3 (7 n/M) > thrombin (1 U/ml) ≥ ATP (500 μM) > bradykinin (20 μM) ≥ carbachol (500 μM), as indicated by RVD rate constants. The extent of PI hydrolysis induced by these agents at the beginning of RVD exhibited a logarithmic relationship with the magnitude of RVD enhancement. Also, there was a linear relationship between the rate of PI hydrolysis and RVD rate constants. Our results suggest that stimulated PI hydrolysis is involved in the regulation of cell volume in astrocytes.

Original languageEnglish
Pages (from-to)1506-1514
Number of pages9
JournalJournal of Neurochemistry
Volume61
Issue number4
StatePublished - Oct 1 1993

Fingerprint

Phosphatidylinositols
Astrocytes
Hydrolysis
Rate constants
Endothelin-3
Endothelins
Carbachol
Type C Phospholipases
Bradykinin
Cell Size
Thrombin
Swelling
Norepinephrine
Adenosine Triphosphate
Cells

Keywords

  • Astrocytes
  • Cell volume
  • Inositol phosphates
  • Intracellular free Ca
  • Phosphatidylinositol hydrolysis agonists
  • Regulatory volume decrease

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Role of phosphoinositide hydrolysis in astrocyte volume regulation. / Bender, A. S.; Neary, J. T.; Norenberg, Michael D.

In: Journal of Neurochemistry, Vol. 61, No. 4, 01.10.1993, p. 1506-1514.

Research output: Contribution to journalArticle

Bender, A. S. ; Neary, J. T. ; Norenberg, Michael D. / Role of phosphoinositide hydrolysis in astrocyte volume regulation. In: Journal of Neurochemistry. 1993 ; Vol. 61, No. 4. pp. 1506-1514.
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