Role of p19ink4d in the pathogenesis of hearing loss

Ruosha Lai, Jingkun Li, Peng Hu, Jie Wen, Qing Jie, Yunpeng Dong, Tao Peng, Xue Z Liu, Dinghua Xie

Research output: Contribution to journalArticle

Abstract

This study aimed to investigate the p19 expression in cisplatin-treated rats and the role of p19 in the degeneration of inner ear cells. It also searched for p19 gene alterations in patients with profound sensorineural deafness. P19ink4d is essential for the postmitotic maintenance of hair cells. It is presumed that a mutation in the functional homolog of p19 or a disturbance in its regulated expression can be the underlying cause of hearing loss. Experiments were conducted on male and female Sprague-Dawley rats (aged 6-7 weeks, 280-320 g) with thresholds of auditory brainstem responses < 30 dB in the sound pressure level, and signs of middle ear infection were used for the experiment. For clinical evaluation, 400 children (age less than 13 years) from unrelated families with severe or profound sensorineural hearing loss (SNHL) were recruited at the second Xiangya Hospital of Central South University between 2005 and 2013, and genomic DNA for deafness gene analysis was obtained from peripheral blood samples of the patients and their lineal relatives. It was found that the p19 expression increased over time in the inner ear cells after cisplatin administration, but the p19 mRNA and protein levels significantly decreased in rats with manifested hearing loss induced by cisplatin. However, no mutation existed within the coding exons of p19 in the patients with profound sensorineural deafness. To conclude, the results support the concept that p19 may play an important role in the ototoxic effects of cisplatin and is probably involved in the pathogenesis of hearing loss.

Original languageEnglish (US)
Pages (from-to)12243-12251
Number of pages9
JournalInternational Journal of Clinical and Experimental Pathology
Volume8
Issue number10
StatePublished - 2015

Fingerprint

Hearing Loss
Cisplatin
Deafness
Inner Ear
Mutation
Brain Stem Auditory Evoked Potentials
Sensorineural Hearing Loss
Middle Ear
Genes
Sprague Dawley Rats
Exons
Maintenance
Pressure
Messenger RNA
DNA
Infection
Proteins

Keywords

  • Hearing loss
  • Inner ear cells
  • P19ink4d

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Lai, R., Li, J., Hu, P., Wen, J., Jie, Q., Dong, Y., ... Xie, D. (2015). Role of p19ink4d in the pathogenesis of hearing loss. International Journal of Clinical and Experimental Pathology, 8(10), 12243-12251.

Role of p19ink4d in the pathogenesis of hearing loss. / Lai, Ruosha; Li, Jingkun; Hu, Peng; Wen, Jie; Jie, Qing; Dong, Yunpeng; Peng, Tao; Liu, Xue Z; Xie, Dinghua.

In: International Journal of Clinical and Experimental Pathology, Vol. 8, No. 10, 2015, p. 12243-12251.

Research output: Contribution to journalArticle

Lai, R, Li, J, Hu, P, Wen, J, Jie, Q, Dong, Y, Peng, T, Liu, XZ & Xie, D 2015, 'Role of p19ink4d in the pathogenesis of hearing loss', International Journal of Clinical and Experimental Pathology, vol. 8, no. 10, pp. 12243-12251.
Lai, Ruosha ; Li, Jingkun ; Hu, Peng ; Wen, Jie ; Jie, Qing ; Dong, Yunpeng ; Peng, Tao ; Liu, Xue Z ; Xie, Dinghua. / Role of p19ink4d in the pathogenesis of hearing loss. In: International Journal of Clinical and Experimental Pathology. 2015 ; Vol. 8, No. 10. pp. 12243-12251.
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