Role of nitric oxide in the cerebrovascular and thermoregulatory response to interleukin-1β

Manuel Monroy, John W. Kuluz, Dansha He, W. Dalton Dietrich, Charles L. Schleien

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Central administration of interleukin-1β (IL-1β) increases cerebral blood flow (CBF) and body temperature, in part, through the production of prostaglandins. In previous studies, the temporal relationship between these effects of IL-1β have not been measured. In this study, we hypothesized that the increase in CBF occurs before any change in brain or body temperature and that the cerebrovascular and thermoregulatory effects of IL-1β would be attenuated by inhibiting the production of nitric oxide (NO). Adult male rats received 100 ng intracerebroventricular (icv) injection of IL-1β, and cortical CBF (cCBF) was measured by laser-Doppler in the contralateral cerebral cortex. A central injection of IL-1β caused a rapid increase in cCBF to 133 ± 12% of baseline within 15 min and to an average of 137 ± 12% for the remainder of the 3-h experiment. Brain and rectal temperature increased by 0.4 ± 0.2 and 0.5 ± 0.2°C, but not until 45 min after IL-1β administration. Pretreatment with Nω-nitro-L-arginine methyl ester (L-NAME; 5 mg/kg iv) completely prevented the changes in cCBF and brain and recta/ temperature induced by IL-1β. L-Arginine (150 mg/kg iv) partially reversed the effects of L-NAME and resulted in increases in both cCBF and temperature. These findings suggest that the vasodilatory effects of IL-1β in the cerebral vasculature are independent of temperature and that NO plays a major role in both the cerebrovascular and thermoregulatory effects of centrally administered IL-1β.

Original languageEnglish (US)
Pages (from-to)H1448-H1453
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number4 49-4
StatePublished - Apr 2001


  • Brain
  • Cerebral blood flow
  • Cytokines
  • Fever
  • Inflammation
  • Temperature
  • Vasodilation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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