Role of neutrophils and macrophages in the pathogenesis of necrotizing enterocolitis caused by Cronobacter sakazakii

Claudia N. Emami, Rahul Mittal, Larry Wang, Henri Ford, Nemani V. Prasadarao

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Cronobacter sakazakii (CS) is a highly virulent gram-negative opportunistic pathogen that has been implicated in clinical outbreaks of necrotizing enterocolitis (NEC). The role of mucosal immune cells in CS infection is not well understood. In this study, we sought to elucidate the role of neutrophils (polymorphonuclear leukocytes; PMNs) and macrophages in the pathogenesis of NEC induced by CS using a novel newborn mouse model. Materials and Methods: PMNs and macrophages were depleted in newborn mice using Gr-1 antibody and carrageenan, respectively, and then infected with 10 3 CFU of CS. The development of NEC in these mice was assessed by a pathologist based on the morphologic changes in the intestine. Cytokine production was determined in the serum and intestinal homogenates of infected mice by enzyme-linked immunosorbent assay (ELISA). Inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production was determined by flow cytometry and Greiss method, respectively. Results: Depletion of PMNs and macrophages in newborn mice led to increased recruitment of dendritic cells (DCs) in the intestine compared with wild-type mice upon infection with CS. PMN- and macrophage-depleted mice showed increased bacterial load, production of pro-inflammatory cytokines, iNOS expression, and NO production in the intestines in comparison to wild-type mice fed with CS. In addition, depletion of PMNs and macrophages prior to infection in mice resulted in severe inflammation, villus destruction, and enhanced enterocyte apoptosis in the intestines compared with CS-infected wild-type mice. Conclusions: Our data suggest that depletion of PMNs and macrophages from the lamina propria (LP) exacerbates experimental NEC, indicating that both of these immunocytes play an important role in the clearance of CS during the initial stages of infection. The increased mucosal cytokine response and NO production in the absence of these immunocytes may be responsible for the observed increase in mucosal injury. Understanding how CS manipulates these cells, employing novel mouse model of NEC reported in this study, will provide significant insights for the development of novel therapeutic and preventive strategies to combat NEC.

Original languageEnglish (US)
Pages (from-to)18-28
Number of pages11
JournalJournal of Surgical Research
Volume172
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Fingerprint

Cronobacter sakazakii
Necrotizing Enterocolitis
Neutrophils
Macrophages
Intestines
Nitric Oxide
Nitric Oxide Synthase Type II
Cytokines
Infection
Bacterial Load
Enterocytes
Carrageenan
Dendritic Cells
Disease Outbreaks

Keywords

  • Cronobacter sakazakii
  • dendritic cells
  • macrophages
  • necrotizing enterocolitis
  • nitric oxide
  • PMNs

ASJC Scopus subject areas

  • Surgery

Cite this

Role of neutrophils and macrophages in the pathogenesis of necrotizing enterocolitis caused by Cronobacter sakazakii. / Emami, Claudia N.; Mittal, Rahul; Wang, Larry; Ford, Henri; Prasadarao, Nemani V.

In: Journal of Surgical Research, Vol. 172, No. 1, 01.01.2012, p. 18-28.

Research output: Contribution to journalArticle

Emami, Claudia N. ; Mittal, Rahul ; Wang, Larry ; Ford, Henri ; Prasadarao, Nemani V. / Role of neutrophils and macrophages in the pathogenesis of necrotizing enterocolitis caused by Cronobacter sakazakii. In: Journal of Surgical Research. 2012 ; Vol. 172, No. 1. pp. 18-28.
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