Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl

Gian Franco Sferrazza, Chi Zhang, Pingping Jia, Sharon L. Lemanski, Gagani Athauda, Alyssa Stassi, Kristine Halager, Jennifer A. Maier, Elena Rueda-de-Leon, Amit Gupta, Syamalima Dube, Xupei Huang, Howard M. Prentice, Dipak K. Dube, Larry F. Lemanski

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Abstract

The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene ``c", for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts. Myofibril-inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac troponin T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalBiochemical and biophysical research communications
Volume357
Issue number1
DOIs
StatePublished - May 25 2007

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Keywords

  • Alternative splicing
  • Axolotl
  • Cardiac troponin T
  • Myofibril

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sferrazza, G. F., Zhang, C., Jia, P., Lemanski, S. L., Athauda, G., Stassi, A., Halager, K., Maier, J. A., Rueda-de-Leon, E., Gupta, A., Dube, S., Huang, X., Prentice, H. M., Dube, D. K., & Lemanski, L. F. (2007). Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl. Biochemical and biophysical research communications, 357(1), 32-37. https://doi.org/10.1016/j.bbrc.2007.03.064