Abstract
Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.
| Original language | English |
|---|---|
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 6 |
| State | Published - Dec 1 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
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Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection. / Qvaizu, N.; Adachi, Y.; McCloskey, T. W.; Than, S.; Lakshmi, T.; Hosaka, N.; Gonaradawann, H.; Yagita, H.; Pahwa, Savita G.
In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection
AU - Qvaizu, N.
AU - Adachi, Y.
AU - McCloskey, T. W.
AU - Than, S.
AU - Lakshmi, T.
AU - Hosaka, N.
AU - Gonaradawann, H.
AU - Yagita, H.
AU - Pahwa, Savita G
PY - 1996/12/1
Y1 - 1996/12/1
N2 - Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.
AB - Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.
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M3 - Article
AN - SCOPUS:33749089480
VL - 10
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 6
ER -