Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection

N. Qvaizu, Y. Adachi, T. W. McCloskey, S. Than, T. Lakshmi, N. Hosaka, H. Gonaradawann, H. Yagita, S. Pahwa

Research output: Contribution to journalArticle

Abstract

Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.

Original languageEnglish (US)
Pages (from-to)A1341
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Qvaizu, N., Adachi, Y., McCloskey, T. W., Than, S., Lakshmi, T., Hosaka, N., Gonaradawann, H., Yagita, H., & Pahwa, S. (1996). Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection. FASEB Journal, 10(6), A1341.