Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection

N. Qvaizu, Y. Adachi, T. W. McCloskey, S. Than, T. Lakshmi, N. Hosaka, H. Gonaradawann, H. Yagita, Savita G Pahwa

Research output: Contribution to journalArticle

Abstract

Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

T-cells
crosslinking
Crosslinking
monocytes
Monocytes
apoptosis
T-lymphocytes
Apoptosis
T-Lymphocytes
Infection
infection
Fas Ligand Protein
cells
Accessories
HIV infections
mononuclear leukocytes
Viruses
B-lymphocytes
HIV Infections
B-Lymphocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Qvaizu, N., Adachi, Y., McCloskey, T. W., Than, S., Lakshmi, T., Hosaka, N., ... Pahwa, S. G. (1996). Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection. FASEB Journal, 10(6).

Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection. / Qvaizu, N.; Adachi, Y.; McCloskey, T. W.; Than, S.; Lakshmi, T.; Hosaka, N.; Gonaradawann, H.; Yagita, H.; Pahwa, Savita G.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticle

Qvaizu, N, Adachi, Y, McCloskey, TW, Than, S, Lakshmi, T, Hosaka, N, Gonaradawann, H, Yagita, H & Pahwa, SG 1996, 'Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection', FASEB Journal, vol. 10, no. 6.
Qvaizu N, Adachi Y, McCloskey TW, Than S, Lakshmi T, Hosaka N et al. Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection. FASEB Journal. 1996 Dec 1;10(6).
Qvaizu, N. ; Adachi, Y. ; McCloskey, T. W. ; Than, S. ; Lakshmi, T. ; Hosaka, N. ; Gonaradawann, H. ; Yagita, H. ; Pahwa, Savita G. / Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
@article{e830d0a7e32a4483835e5325ca7c964a,
title = "Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection",
abstract = "Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.",
author = "N. Qvaizu and Y. Adachi and McCloskey, {T. W.} and S. Than and T. Lakshmi and N. Hosaka and H. Gonaradawann and H. Yagita and Pahwa, {Savita G}",
year = "1996",
month = "12",
day = "1",
language = "English",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - Role of monocytes on cd4 crosslinking-induced t cells apoptosis in hiv infection

AU - Qvaizu, N.

AU - Adachi, Y.

AU - McCloskey, T. W.

AU - Than, S.

AU - Lakshmi, T.

AU - Hosaka, N.

AU - Gonaradawann, H.

AU - Yagita, H.

AU - Pahwa, Savita G

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.

AB - Recent evidence points to an important immunopathogenic role of monocytes in HIV infection as reservoirs of the virus as well as T cell apoptosis-inducers. However, the mechanisms by which monocytes induce T cell apoptosis is ill-defined. We have previously demonstrated that CD4 cross-linking (XL) performed in PBMC resulted in T cell apoptosis induction in an accessory cell-dependent manner. In this study, we examined the role of monocytes in a CD4XL-induced T cell apoptosis system with special reference to interaction of Fas and its ligand (FasL). Here we report that i) depletion of monocytes, but not of B cells or CD8+ T cells, abrogates CD4XL-induced T cell apoptosis; conversely, addition of monocytes to purified CD4+ T cells leads to apoptosis; il) CD4XL performed in purified CD4+ T cells upregulates Fas, but not FasL expression. We favor the hypothesis that CD4XL leads to FasL induction in monocyte and results in bystander T cell apoptosis via a mechanism of FasFasL interaction.

UR - http://www.scopus.com/inward/record.url?scp=33749089480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749089480&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749089480

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -