Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies

Thomas Malek, G. Ortega, C. Chan, R. A. Kroczek, E. M. Shevach

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Abstract

The Ly-6 locus controls the expression and/or encodes for alloantigenic specificities found primarily on subpopulations of murine T and B lymphocytes. We have recently identified and characterized a new rat mAb, D7, that recognizes a nonpolymorphic Ly-6 specificity. After crosslinking by anti-Ig reagents or by Fc receptor-bearing accessory cells, mAb D7 could induce IL-2 production from T cell hybridomas, and in the presence of PMA could trigger a vigorous proliferative response in resting peripheral T cells. The addition of mAb D7 to cultures of antigen- and alloantigen-, but not mitogen-stimulated T cells resulted in a marked augmentation of the proliferative response. A number of other well-characterized mAbs to Ly-6 locus products could also stimulate a T cell proliferative response after crosslinking by anti-Ig and in the presence of PMA. These results strongly suggest that Ly-6 molecules may play a critical role in the T cell activation cascade, either as receptors for an unidentified soluble or cell-associated ligand or as transducing molecules that modulate signals initiated by antigen stimulation of the T3-Ti complex.

Original languageEnglish
Pages (from-to)709-722
Number of pages14
JournalJournal of Experimental Medicine
Volume164
Issue number3
StatePublished - Dec 3 1986

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Lymphocyte Activation
T-Lymphocytes
Antibodies
CD3 Antigens
Antigens
Internal-External Control
Isoantigens
Fc Receptors
Hybridomas
Mitogens
Interleukin-2
B-Lymphocytes
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies. / Malek, Thomas; Ortega, G.; Chan, C.; Kroczek, R. A.; Shevach, E. M.

In: Journal of Experimental Medicine, Vol. 164, No. 3, 03.12.1986, p. 709-722.

Research output: Contribution to journalArticle

Malek, Thomas ; Ortega, G. ; Chan, C. ; Kroczek, R. A. ; Shevach, E. M. / Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies. In: Journal of Experimental Medicine. 1986 ; Vol. 164, No. 3. pp. 709-722.
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