Role of interleukin 12 and costimulators in T cell anergy in vivo

Luk Van Parijs, Victor L Perez Quinones, Andre Biuckians, Robert G. Maki, Cheryl A. London, Abul K. Abbas

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The induction oft cell anergy in vivo is thought to result from antigen recognition in the absence of co-stimulation and inflammation, and is associated with a block in T cell proliferation and Th1 differentiation. Here we have examined the role of interleukin (IL)-12, a potent inducer of Th1 responses, in regulating this process. T cell tolerance was induced by the administration of protein antigen without adjuvant in normal mice, and in recipients of adoptively transferred T cells from T cell receptor transgenic mice. The administration of IL-12 at the time of tolerance induction stimulates Th1 differentiation, but does not promote antigen-specific T cell proliferation. Conversely, inhibiting CTLA-4 engagement during anergy induction reverses the block in T cell proliferation, but does not promote full Th1 differentiation. T cells exposed to tolerogenic antigen in the presence of both IL-12 and anti-CTLA-4 antibody are not anergized, and behave identically to T cells which have encountered immunogenic antigen. These results suggest that two processes contribute to the induction of anergy in vivo; CTLA-4 engagement, which leads to a block in the ability oft cells to proliferate to antigen, and the absence of a prototypic inflammatory cytokine, IL-12, which prevents the differentiation oft cells into Th1 effector cells. The combination of IL-12 and anti-CTLA-4 antibody is sufficient to convert a normally tolerogenic stimulus to an immunogenic one.

Original languageEnglish
Pages (from-to)1119-1128
Number of pages10
JournalJournal of Experimental Medicine
Volume186
Issue number7
DOIs
StatePublished - Oct 6 1997
Externally publishedYes

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Interleukin-12
T-Lymphocytes
Antigens
Cell Proliferation
Th1 Cells
Antibodies
T-Cell Antigen Receptor
Transgenic Mice
Cell Differentiation
Cytokines
Inflammation
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Van Parijs, L., Perez Quinones, V. L., Biuckians, A., Maki, R. G., London, C. A., & Abbas, A. K. (1997). Role of interleukin 12 and costimulators in T cell anergy in vivo. Journal of Experimental Medicine, 186(7), 1119-1128. https://doi.org/10.1084/jem.186.7.1119

Role of interleukin 12 and costimulators in T cell anergy in vivo. / Van Parijs, Luk; Perez Quinones, Victor L; Biuckians, Andre; Maki, Robert G.; London, Cheryl A.; Abbas, Abul K.

In: Journal of Experimental Medicine, Vol. 186, No. 7, 06.10.1997, p. 1119-1128.

Research output: Contribution to journalArticle

Van Parijs, L, Perez Quinones, VL, Biuckians, A, Maki, RG, London, CA & Abbas, AK 1997, 'Role of interleukin 12 and costimulators in T cell anergy in vivo', Journal of Experimental Medicine, vol. 186, no. 7, pp. 1119-1128. https://doi.org/10.1084/jem.186.7.1119
Van Parijs L, Perez Quinones VL, Biuckians A, Maki RG, London CA, Abbas AK. Role of interleukin 12 and costimulators in T cell anergy in vivo. Journal of Experimental Medicine. 1997 Oct 6;186(7):1119-1128. https://doi.org/10.1084/jem.186.7.1119
Van Parijs, Luk ; Perez Quinones, Victor L ; Biuckians, Andre ; Maki, Robert G. ; London, Cheryl A. ; Abbas, Abul K. / Role of interleukin 12 and costimulators in T cell anergy in vivo. In: Journal of Experimental Medicine. 1997 ; Vol. 186, No. 7. pp. 1119-1128.
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