Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation

Jean Baptiste Demoulin; Luigi Grasso; John M. Atkins; Monique Stevens; Jamila Louahed; Roy C. Levitt; Nicholas C. Nicolaides; Jean Christophe Renauld

doi:10.1016/S0014-5793(00)02059-7

Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation

Jean Baptiste Demoulin, Luigi Grasso, John M. Atkins, Monique Stevens, Jamila Louahed, Roy C. Levitt, Nicholas C. Nicolaides, Jean Christophe Renauld

Research output: Contribution to journal › Article

15 Scopus citations

Abstract

Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	200-204
Number of pages	5
Journal	FEBS letters
Volume	482
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(00)02059-7
State	Published - Oct 6 2000
Externally published	Yes

Keywords

Apoptosis
Insulin receptor substrate
Interleukin-9
Proliferation
Protein kinase B

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Demoulin, J. B., Grasso, L., Atkins, J. M., Stevens, M., Louahed, J., Levitt, R. C., Nicolaides, N. C., & Renauld, J. C. (2000). Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation. FEBS letters, 482(3), 200-204. https://doi.org/10.1016/S0014-5793(00)02059-7

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title = "Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation",

abstract = "Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.",

keywords = "Apoptosis, Insulin receptor substrate, Interleukin-9, Proliferation, Protein kinase B",

author = "Demoulin, {Jean Baptiste} and Luigi Grasso and Atkins, {John M.} and Monique Stevens and Jamila Louahed and Levitt, {Roy C.} and Nicolaides, {Nicholas C.} and Renauld, {Jean Christophe}",

year = "2000",

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doi = "10.1016/S0014-5793(00)02059-7",

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AU - Demoulin, Jean Baptiste

AU - Grasso, Luigi

AU - Atkins, John M.

AU - Stevens, Monique

AU - Louahed, Jamila

AU - Levitt, Roy C.

AU - Nicolaides, Nicholas C.

AU - Renauld, Jean Christophe

PY - 2000/10/6

Y1 - 2000/10/6

N2 - Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

AB - Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

KW - Apoptosis

KW - Insulin receptor substrate

KW - Interleukin-9

KW - Proliferation

KW - Protein kinase B

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