Abstract
Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 200-204 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 482 |
| Issue number | 3 |
| DOIs | |
| State | Published - Oct 6 2000 |
| Externally published | Yes |
Fingerprint
Keywords
- Apoptosis
- Insulin receptor substrate
- Interleukin-9
- Proliferation
- Protein kinase B
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation. / Demoulin, Jean Baptiste; Grasso, Luigi; Atkins, John M.; Stevens, Monique; Louahed, Jamila; Levitt, Roy C; Nicolaides, Nicholas C.; Renauld, Jean Christophe.
In: FEBS Letters, Vol. 482, No. 3, 06.10.2000, p. 200-204.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation
AU - Demoulin, Jean Baptiste
AU - Grasso, Luigi
AU - Atkins, John M.
AU - Stevens, Monique
AU - Louahed, Jamila
AU - Levitt, Roy C
AU - Nicolaides, Nicholas C.
AU - Renauld, Jean Christophe
PY - 2000/10/6
Y1 - 2000/10/6
N2 - Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.
AB - Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.
KW - Apoptosis
KW - Insulin receptor substrate
KW - Interleukin-9
KW - Proliferation
KW - Protein kinase B
UR - http://www.scopus.com/inward/record.url?scp=0034613098&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034613098&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(00)02059-7
DO - 10.1016/S0014-5793(00)02059-7
M3 - Article
C2 - 11024460
AN - SCOPUS:0034613098
VL - 482
SP - 200
EP - 204
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -