Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation

Jean Baptiste Demoulin; Luigi Grasso; John M. Atkins; Monique Stevens; Jamila Louahed; Roy C. Levitt; Nicholas C. Nicolaides; Jean Christophe Renauld

doi:10.1016/S0014-5793(00)02059-7

Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation

Jean Baptiste Demoulin, Luigi Grasso, John M. Atkins, Monique Stevens, Jamila Louahed, Roy C. Levitt, Nicholas C. Nicolaides, Jean Christophe Renauld

Anesthesiology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	200-204
Number of pages	5
Journal	FEBS letters
Volume	482
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(00)02059-7
State	Published - Oct 6 2000

Keywords

Apoptosis
Insulin receptor substrate
Interleukin-9
Proliferation
Protein kinase B

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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@article{81ca28cdda7c4e0babb81cd0ab897e60,

title = "Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation",

abstract = "Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.",

keywords = "Apoptosis, Insulin receptor substrate, Interleukin-9, Proliferation, Protein kinase B",

author = "Demoulin, {Jean Baptiste} and Luigi Grasso and Atkins, {John M.} and Monique Stevens and Jamila Louahed and Levitt, {Roy C.} and Nicolaides, {Nicholas C.} and Renauld, {Jean Christophe}",

note = "Funding Information: This work was supported in part by the Belgian Federal Service for Scientific, Technical and Cultural Affairs and by the Actions de Recherche Concert{\'e}es, Communaut{\'e} fran{\c c}aise de Belgique, Direction de la recherche scientifique. J.-B.D. is a senior research assistant and J.-C.R. is a research associate with the Fonds National de la Recherche Scientifique, Belgium. We also thank Minxue Huang for her technical contribution.",

year = "2000",

month = oct,

day = "6",

doi = "10.1016/S0014-5793(00)02059-7",

language = "English (US)",

volume = "482",

pages = "200--204",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation

AU - Demoulin, Jean Baptiste

AU - Grasso, Luigi

AU - Atkins, John M.

AU - Stevens, Monique

AU - Louahed, Jamila

AU - Levitt, Roy C.

AU - Nicolaides, Nicholas C.

AU - Renauld, Jean Christophe

N1 - Funding Information: This work was supported in part by the Belgian Federal Service for Scientific, Technical and Cultural Affairs and by the Actions de Recherche Concertées, Communauté française de Belgique, Direction de la recherche scientifique. J.-B.D. is a senior research assistant and J.-C.R. is a research associate with the Fonds National de la Recherche Scientifique, Belgium. We also thank Minxue Huang for her technical contribution.

PY - 2000/10/6

Y1 - 2000/10/6

N2 - Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

AB - Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

KW - Apoptosis

KW - Insulin receptor substrate

KW - Interleukin-9

KW - Proliferation

KW - Protein kinase B

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