Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation

Jean Baptiste Demoulin, Luigi Grasso, John M. Atkins, Monique Stevens, Jamila Louahed, Roy C Levitt, Nicholas C. Nicolaides, Jean Christophe Renauld

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)200-204
Number of pages5
JournalFEBS Letters
Volume482
Issue number3
DOIs
StatePublished - Oct 6 2000
Externally publishedYes

Fingerprint

Interleukin-9
Insulin Receptor Substrate Proteins
Phosphorylation
Phosphatidylinositol 3-Kinase
Proto-Oncogene Proteins c-akt
Interleukin-9 Receptors
Cells
Association reactions
Cell Line

Keywords

  • Apoptosis
  • Insulin receptor substrate
  • Interleukin-9
  • Proliferation
  • Protein kinase B

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Demoulin, J. B., Grasso, L., Atkins, J. M., Stevens, M., Louahed, J., Levitt, R. C., ... Renauld, J. C. (2000). Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation. FEBS Letters, 482(3), 200-204. https://doi.org/10.1016/S0014-5793(00)02059-7

Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation. / Demoulin, Jean Baptiste; Grasso, Luigi; Atkins, John M.; Stevens, Monique; Louahed, Jamila; Levitt, Roy C; Nicolaides, Nicholas C.; Renauld, Jean Christophe.

In: FEBS Letters, Vol. 482, No. 3, 06.10.2000, p. 200-204.

Research output: Contribution to journalArticle

Demoulin, JB, Grasso, L, Atkins, JM, Stevens, M, Louahed, J, Levitt, RC, Nicolaides, NC & Renauld, JC 2000, 'Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation', FEBS Letters, vol. 482, no. 3, pp. 200-204. https://doi.org/10.1016/S0014-5793(00)02059-7
Demoulin, Jean Baptiste ; Grasso, Luigi ; Atkins, John M. ; Stevens, Monique ; Louahed, Jamila ; Levitt, Roy C ; Nicolaides, Nicholas C. ; Renauld, Jean Christophe. / Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation. In: FEBS Letters. 2000 ; Vol. 482, No. 3. pp. 200-204.
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