Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation

Jean Baptiste Demoulin, Luigi Grasso, John M. Atkins, Monique Stevens, Jamila Louahed, Roy C. Levitt, Nicholas C. Nicolaides, Jean Christophe Renauld

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K- dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)200-204
Number of pages5
JournalFEBS letters
Volume482
Issue number3
DOIs
StatePublished - Oct 6 2000
Externally publishedYes

Keywords

  • Apoptosis
  • Insulin receptor substrate
  • Interleukin-9
  • Proliferation
  • Protein kinase B

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation'. Together they form a unique fingerprint.

  • Cite this

    Demoulin, J. B., Grasso, L., Atkins, J. M., Stevens, M., Louahed, J., Levitt, R. C., Nicolaides, N. C., & Renauld, J. C. (2000). Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation. FEBS letters, 482(3), 200-204. https://doi.org/10.1016/S0014-5793(00)02059-7