Abstract
Mu-opioid receptor knockout mice (MORKO), were used to address two questions: (1) if morphine induced decrease in thymic weight and cell distribution is mediated by the mu-opioid receptor and (2) the role of corticosteroids in morphine mediated alteration in thymic cell distribution. Our result show that morphine mediated increase in plasma corticosterone is mediated by the mu-opioid receptor since morphine at doses as high as 25 mg/kg-body weight does not increase plasma corticosterone levels in the MORKO. In addition, we have also shown that morphine treatment results in the differentiation of CD4 + CD8 + (double positive cells) to single positive CD4 + cells while dexamethasone treatment results in the deletion of CD4 + CD8 + (double positive) cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 147-155 |
| Number of pages | 9 |
| Journal | Journal of Neuroimmunology |
| Volume | 116 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 1 2001 |
| Externally published | Yes |
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Keywords
- Corticosterone
- Dexamethasone
- Morphine
- Mu-opioid receptor knockout
- T cell
- Thymus
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology
Cite this
Role of hypothalamic-pituitary axis in morphine-induced alteration in thymic cell distribution using mu-opioid receptor knockout mice. / Roy, Sabita; Wang, Jing Hua; Balasubramanian, Sudha; Sumandeep; Charboneau, Rick; Barke, Roderick; Loh, Horace H.
In: Journal of Neuroimmunology, Vol. 116, No. 2, 01.06.2001, p. 147-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of hypothalamic-pituitary axis in morphine-induced alteration in thymic cell distribution using mu-opioid receptor knockout mice
AU - Roy, Sabita
AU - Wang, Jing Hua
AU - Balasubramanian, Sudha
AU - Sumandeep,
AU - Charboneau, Rick
AU - Barke, Roderick
AU - Loh, Horace H.
PY - 2001/6/1
Y1 - 2001/6/1
N2 - Mu-opioid receptor knockout mice (MORKO), were used to address two questions: (1) if morphine induced decrease in thymic weight and cell distribution is mediated by the mu-opioid receptor and (2) the role of corticosteroids in morphine mediated alteration in thymic cell distribution. Our result show that morphine mediated increase in plasma corticosterone is mediated by the mu-opioid receptor since morphine at doses as high as 25 mg/kg-body weight does not increase plasma corticosterone levels in the MORKO. In addition, we have also shown that morphine treatment results in the differentiation of CD4 + CD8 + (double positive cells) to single positive CD4 + cells while dexamethasone treatment results in the deletion of CD4 + CD8 + (double positive) cells.
AB - Mu-opioid receptor knockout mice (MORKO), were used to address two questions: (1) if morphine induced decrease in thymic weight and cell distribution is mediated by the mu-opioid receptor and (2) the role of corticosteroids in morphine mediated alteration in thymic cell distribution. Our result show that morphine mediated increase in plasma corticosterone is mediated by the mu-opioid receptor since morphine at doses as high as 25 mg/kg-body weight does not increase plasma corticosterone levels in the MORKO. In addition, we have also shown that morphine treatment results in the differentiation of CD4 + CD8 + (double positive cells) to single positive CD4 + cells while dexamethasone treatment results in the deletion of CD4 + CD8 + (double positive) cells.
KW - Corticosterone
KW - Dexamethasone
KW - Morphine
KW - Mu-opioid receptor knockout
KW - T cell
KW - Thymus
UR - http://www.scopus.com/inward/record.url?scp=0035363736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035363736&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(01)00299-5
DO - 10.1016/S0165-5728(01)00299-5
M3 - Article
C2 - 11438169
AN - SCOPUS:0035363736
VL - 116
SP - 147
EP - 155
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 2
ER -