Role of hydrogen peroxide in experimental optic neuritis. A serial quantitative ultrastructural study

John Guy, E. A. Ellis, R. Mames, N. A. Rao

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In order to determine the role of H2O2 in demyelination of the optic nerve, serial quantitative analysis of H2O2-derived cerium perhydroxide reaction product particles was obtained by computerized digitization of electron micrographs of the myelinated retrobulbar optic nerve, unmyelinated optic nerve head, and the optic nerve sheath of guinea pigs sensitized for experimental allergic encephalomyelitis (EAE) and euthanized 3-14 days later. We found that cerium perhydroxide reaction product particles were greatest in the myelinated optic nerve 3 days after antigenic sensitization, but at this focus decreased 7-14 days after antigenic sensitization. Reaction product accumulated in the unmyelinated optic nerve head and optic nerve sheath 3-14 days after sensitization. These results in the myelinated optic nerve suggest H2O2 consumption results in peroxidation of myelin lipid as demyelination proceeds 7-14 days after antigenic sensitization. Hydrogen peroxide accumulation in the optic nerve head and the optic nerve sheath appears to provide a reservoir for diffusion of H2O2 into the retrobulbar optic nerve and adjacent perineural nerve, contributing to the frequent predilection for optic nerve involvement in EAE and perhaps in multiple sclerosis.

Original languageEnglish
Pages (from-to)253-264
Number of pages12
JournalOphthalmic Research
Volume25
Issue number4
StatePublished - Sep 12 1993
Externally publishedYes

Fingerprint

Optic Neuritis
Optic Nerve
Hydrogen Peroxide
Optic Disk
Autoimmune Experimental Encephalomyelitis
Demyelinating Diseases
Myelin Sheath
Lipid Peroxidation
Multiple Sclerosis
Guinea Pigs
Electrons

Keywords

  • Experimental allergic encephalomyelitis
  • Free oxygen radicals
  • Hydrogen peroxide
  • Multiple sclerosis
  • Optic neuritis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Role of hydrogen peroxide in experimental optic neuritis. A serial quantitative ultrastructural study. / Guy, John; Ellis, E. A.; Mames, R.; Rao, N. A.

In: Ophthalmic Research, Vol. 25, No. 4, 12.09.1993, p. 253-264.

Research output: Contribution to journalArticle

Guy, John ; Ellis, E. A. ; Mames, R. ; Rao, N. A. / Role of hydrogen peroxide in experimental optic neuritis. A serial quantitative ultrastructural study. In: Ophthalmic Research. 1993 ; Vol. 25, No. 4. pp. 253-264.
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