Role of group-specific component (vitamin D binding protein) in clearance of actin from the circulation in the rabbit

P. J. Goldschmidt-Clermont, H. Van Baelen, R. Bouillon, T. E. Shook, M. H. Williams, A. E. Nel, R. M. Galbraith

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

The possible role of group specific component (Gc) (vitamin D-binding protein) in the clearance of cellular actin entering the circulation was examined with 125I-labeled Gc and actin injected into a rabbit model. Although filamentous F-actin is depolymerized primarily by plasma gelsolin, ≥ 90% 125I-actin injected in either monomeric G- or F-form became complexed eventually with Gc (1:1 molar ratio). Clearance of Gc complexes was much faster (> 90% within 5 h) than that of native Gc (t 1/2 = 17.2 h). Nephrectomy did not significantly alter the clearance of either Gc or actin. Since Gc complexes are dramatically increased in situations of tissue necrosis such as in fulminant hepatic failure, the current results suggest a crucial role for Gc in sequestration and clearance of released cellular actin.

Original languageEnglish (US)
Pages (from-to)1519-1527
Number of pages9
JournalJournal of Clinical Investigation
Volume81
Issue number5
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Medicine(all)

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