role of cytosolic free calcium concentration in the secretion of calcitonin gene-related peptide and calcitonin from medullary thyroid carcinoma cells

S. Haller-Brem, R. Muff, J. B. Petermann, W. Born, B. A. Roos, J. A. Fischer

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) and calcitonin (CT) are secreted by medullary thyroid carcinoma (MTC). Relationships between extracellular calcium ([Ca2+](e)), cytosolic free calcium ([Ca2+](i)) (as measured with fura-2), and secretion of immunoreactive CGRP and CT have been investigated in rat and human MTC cell lines. Rat MTC 6-23 cells responded to a rise in [Ca2+](e) from 0.5 to 3.0 mM with a transient increase of [Ca2+](i), and the secretion of CGRP and CT was raised from 19 ± 2 (mean ± SE) to 122 ± 28 pg rat CGRP/mg protein·min and from 33 ± 8 to 155 ± 42 pg rat CT/Mg protein·min (P < 0.01). In the human MTC (TT) cell line, a rise of [Ca2+](e) from 0.5 to 3.0 mM did not affect [Ca2+](i), and the secretion of CGRP and CT remained unchanged at 7.0 ± 1.1 ng CGRP/mg protein·min and 1.0 ± 0.1 ng CT/mg protein·min. However, when the plasma membrane was bypassed by electropermeabilization, the release of CGRP and CT was stimulated by calcium with an ED50 of 0.5 μM and 0.3 μM, respectively. With ionomycin, the secretion of CGRP and CT was also stimulated up to 17-fold in [Ca2+](i)-dependent manner. The results indicate a role of [Ca2+](i) in the secretion of CGRP and CT and provide evidence for a defect in Ca2+ signal transduction in the human MTC cell line.

Original languageEnglish
Pages (from-to)1272-1277
Number of pages6
JournalEndocrinology
Volume121
Issue number4
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Calcitonin Gene-Related Peptide
Calcitonin
Calcium
Cell Line
Medullary Thyroid cancer
Ionomycin
Electroporation
Fura-2
Signal Transduction
Cell Membrane

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Haller-Brem, S., Muff, R., Petermann, J. B., Born, W., Roos, B. A., & Fischer, J. A. (1987). role of cytosolic free calcium concentration in the secretion of calcitonin gene-related peptide and calcitonin from medullary thyroid carcinoma cells. Endocrinology, 121(4), 1272-1277.

role of cytosolic free calcium concentration in the secretion of calcitonin gene-related peptide and calcitonin from medullary thyroid carcinoma cells. / Haller-Brem, S.; Muff, R.; Petermann, J. B.; Born, W.; Roos, B. A.; Fischer, J. A.

In: Endocrinology, Vol. 121, No. 4, 01.01.1987, p. 1272-1277.

Research output: Contribution to journalArticle

Haller-Brem, S, Muff, R, Petermann, JB, Born, W, Roos, BA & Fischer, JA 1987, 'role of cytosolic free calcium concentration in the secretion of calcitonin gene-related peptide and calcitonin from medullary thyroid carcinoma cells', Endocrinology, vol. 121, no. 4, pp. 1272-1277.
Haller-Brem, S. ; Muff, R. ; Petermann, J. B. ; Born, W. ; Roos, B. A. ; Fischer, J. A. / role of cytosolic free calcium concentration in the secretion of calcitonin gene-related peptide and calcitonin from medullary thyroid carcinoma cells. In: Endocrinology. 1987 ; Vol. 121, No. 4. pp. 1272-1277.
@article{b203ac03d4854bc3ae75a267ef0009a4,
title = "role of cytosolic free calcium concentration in the secretion of calcitonin gene-related peptide and calcitonin from medullary thyroid carcinoma cells",
abstract = "Calcitonin gene-related peptide (CGRP) and calcitonin (CT) are secreted by medullary thyroid carcinoma (MTC). Relationships between extracellular calcium ([Ca2+](e)), cytosolic free calcium ([Ca2+](i)) (as measured with fura-2), and secretion of immunoreactive CGRP and CT have been investigated in rat and human MTC cell lines. Rat MTC 6-23 cells responded to a rise in [Ca2+](e) from 0.5 to 3.0 mM with a transient increase of [Ca2+](i), and the secretion of CGRP and CT was raised from 19 ± 2 (mean ± SE) to 122 ± 28 pg rat CGRP/mg protein·min and from 33 ± 8 to 155 ± 42 pg rat CT/Mg protein·min (P < 0.01). In the human MTC (TT) cell line, a rise of [Ca2+](e) from 0.5 to 3.0 mM did not affect [Ca2+](i), and the secretion of CGRP and CT remained unchanged at 7.0 ± 1.1 ng CGRP/mg protein·min and 1.0 ± 0.1 ng CT/mg protein·min. However, when the plasma membrane was bypassed by electropermeabilization, the release of CGRP and CT was stimulated by calcium with an ED50 of 0.5 μM and 0.3 μM, respectively. With ionomycin, the secretion of CGRP and CT was also stimulated up to 17-fold in [Ca2+](i)-dependent manner. The results indicate a role of [Ca2+](i) in the secretion of CGRP and CT and provide evidence for a defect in Ca2+ signal transduction in the human MTC cell line.",
author = "S. Haller-Brem and R. Muff and Petermann, {J. B.} and W. Born and Roos, {B. A.} and Fischer, {J. A.}",
year = "1987",
month = "1",
day = "1",
language = "English",
volume = "121",
pages = "1272--1277",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - role of cytosolic free calcium concentration in the secretion of calcitonin gene-related peptide and calcitonin from medullary thyroid carcinoma cells

AU - Haller-Brem, S.

AU - Muff, R.

AU - Petermann, J. B.

AU - Born, W.

AU - Roos, B. A.

AU - Fischer, J. A.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Calcitonin gene-related peptide (CGRP) and calcitonin (CT) are secreted by medullary thyroid carcinoma (MTC). Relationships between extracellular calcium ([Ca2+](e)), cytosolic free calcium ([Ca2+](i)) (as measured with fura-2), and secretion of immunoreactive CGRP and CT have been investigated in rat and human MTC cell lines. Rat MTC 6-23 cells responded to a rise in [Ca2+](e) from 0.5 to 3.0 mM with a transient increase of [Ca2+](i), and the secretion of CGRP and CT was raised from 19 ± 2 (mean ± SE) to 122 ± 28 pg rat CGRP/mg protein·min and from 33 ± 8 to 155 ± 42 pg rat CT/Mg protein·min (P < 0.01). In the human MTC (TT) cell line, a rise of [Ca2+](e) from 0.5 to 3.0 mM did not affect [Ca2+](i), and the secretion of CGRP and CT remained unchanged at 7.0 ± 1.1 ng CGRP/mg protein·min and 1.0 ± 0.1 ng CT/mg protein·min. However, when the plasma membrane was bypassed by electropermeabilization, the release of CGRP and CT was stimulated by calcium with an ED50 of 0.5 μM and 0.3 μM, respectively. With ionomycin, the secretion of CGRP and CT was also stimulated up to 17-fold in [Ca2+](i)-dependent manner. The results indicate a role of [Ca2+](i) in the secretion of CGRP and CT and provide evidence for a defect in Ca2+ signal transduction in the human MTC cell line.

AB - Calcitonin gene-related peptide (CGRP) and calcitonin (CT) are secreted by medullary thyroid carcinoma (MTC). Relationships between extracellular calcium ([Ca2+](e)), cytosolic free calcium ([Ca2+](i)) (as measured with fura-2), and secretion of immunoreactive CGRP and CT have been investigated in rat and human MTC cell lines. Rat MTC 6-23 cells responded to a rise in [Ca2+](e) from 0.5 to 3.0 mM with a transient increase of [Ca2+](i), and the secretion of CGRP and CT was raised from 19 ± 2 (mean ± SE) to 122 ± 28 pg rat CGRP/mg protein·min and from 33 ± 8 to 155 ± 42 pg rat CT/Mg protein·min (P < 0.01). In the human MTC (TT) cell line, a rise of [Ca2+](e) from 0.5 to 3.0 mM did not affect [Ca2+](i), and the secretion of CGRP and CT remained unchanged at 7.0 ± 1.1 ng CGRP/mg protein·min and 1.0 ± 0.1 ng CT/mg protein·min. However, when the plasma membrane was bypassed by electropermeabilization, the release of CGRP and CT was stimulated by calcium with an ED50 of 0.5 μM and 0.3 μM, respectively. With ionomycin, the secretion of CGRP and CT was also stimulated up to 17-fold in [Ca2+](i)-dependent manner. The results indicate a role of [Ca2+](i) in the secretion of CGRP and CT and provide evidence for a defect in Ca2+ signal transduction in the human MTC cell line.

UR - http://www.scopus.com/inward/record.url?scp=0023200546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023200546&partnerID=8YFLogxK

M3 - Article

C2 - 3498624

AN - SCOPUS:0023200546

VL - 121

SP - 1272

EP - 1277

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 4

ER -