Role of CD2 and IL 1β in T cell responsiveness to IL 2

G. Gromo, R. L. Geller, L. Inverardi, S. L. Wee, F. H. Bach

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8 Scopus citations

Abstract

We have evaluated the effects of an anti-CD2 monoclonal antibody (moAb), Leu-5b, on inhibition of lymphocyte responsiveness. Given previous reports that anti-CD2 moAb prevent production of interleukin 2 (IL 2), as well as up-regulation of IL 2 receptor, we chose a test system in which lymphocytes were activated by exogenously added recombinant IL 2 (rIL 2). Our studies thus allowed us to evaluate other possible mechanisms by which anti-CD2 moAb could act. We found that Leu-5b not only blocked the proliferative response of human peripheral blood leukocytes (PBL) to IL 2, but also the development of cytotoxicity as assayed in lectin-dependent cytotoxicity (LDCC). Addition of recombinant interleukin 1β (rIL 1β) reconstituted the proliferative response in the presence of Leu-5b but did not allow development of cytotoxicity. Our findings are consistent with the concept that Leu-5b delivers a signal to IL 2-responsive cells that prevents their subsequent proliferation and development of cytotoxicity, but that only the former event, i.e., proliferation, is reconstituted by the addition of IL 1β.

Original languageEnglish (US)
Pages (from-to)2155-2160
Number of pages6
JournalJournal of Immunology
Volume138
Issue number7
StatePublished - May 27 1987

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Gromo, G., Geller, R. L., Inverardi, L., Wee, S. L., & Bach, F. H. (1987). Role of CD2 and IL 1β in T cell responsiveness to IL 2. Journal of Immunology, 138(7), 2155-2160.