Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls

U. S. Ryan, D. R. Schultz, J. D. Goodwin, J. M. Vann, M. P. Selvaraj, M. A. Hart

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The Re mutant of Salmonella minnesota adheres in much greater numbers than the wild type to endothelial cells derived from the bovine pulmonary artery. Since the Re mutant is distinguished from wild-type S. minnesota by its ability to bind C1q and since endothelial cells possess receptors for C1q, we examined the role of C1q in the phagocytosis of the S. minnesota Re mutant. First, preincubating endothelial cells with C1q-enriched medium resulted in increased adherence of the Re mutant (17.9 x 104 versus 6.6 x 104). Second, preincubating the Re mutant with C1q-enriched medium resulted in increased numbers of adherent bacteria (62.1 x 104 versus 6.6 x 104). Preincubation of both endothelial cells and bacteria with C1q-enriched medium resulted in increased adherence above control levels but less adherence than when either cells or bacteria were preincubated separately in C1q-enriched medium. If serum depleted of C1q was used for preincubation of endothelial cells or bacteria, adherence was reduced below control levels. Thus, C1q plays an important role in the initial steps (recognition, binding, and ingestion) of phagocytosis. Next, the role of C1q was investigated in the respiratory burst response. Levels of superoxide anion released from endothelial cells 15 min after phagocytosis of the Re mutant (100 bacteria per endothelial cell) were assayed by measurement of the superoxide dismutase-inhibitable reduction of ferricytochrome c. Superoxide anion release was increased during phagocytosis of the Re mutant (35 nmol of O2- per 3 x 106 endothelial cells) and was also elevated above control values by incubation with soluble C1q (10 nmol of O2- per 3 x 106 endothelial cells). These results indicate a role for C1q in both the ingestion and the response of endothelial cells to the S. minnesota Re mutant.

Original languageEnglish
Pages (from-to)1356-1362
Number of pages7
JournalInfection and Immunity
Volume57
Issue number5
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Phagocytosis
Salmonella
Endothelial Cells
Lung
Bacteria
Superoxides
Eating
Respiratory Burst
Cytochromes c
Pulmonary Artery
Superoxide Dismutase
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Ryan, U. S., Schultz, D. R., Goodwin, J. D., Vann, J. M., Selvaraj, M. P., & Hart, M. A. (1989). Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls. Infection and Immunity, 57(5), 1356-1362.

Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls. / Ryan, U. S.; Schultz, D. R.; Goodwin, J. D.; Vann, J. M.; Selvaraj, M. P.; Hart, M. A.

In: Infection and Immunity, Vol. 57, No. 5, 01.01.1989, p. 1356-1362.

Research output: Contribution to journalArticle

Ryan, US, Schultz, DR, Goodwin, JD, Vann, JM, Selvaraj, MP & Hart, MA 1989, 'Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls', Infection and Immunity, vol. 57, no. 5, pp. 1356-1362.
Ryan US, Schultz DR, Goodwin JD, Vann JM, Selvaraj MP, Hart MA. Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls. Infection and Immunity. 1989 Jan 1;57(5):1356-1362.
Ryan, U. S. ; Schultz, D. R. ; Goodwin, J. D. ; Vann, J. M. ; Selvaraj, M. P. ; Hart, M. A. / Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls. In: Infection and Immunity. 1989 ; Vol. 57, No. 5. pp. 1356-1362.
@article{605536fa435c455db864cdd4e65a12f4,
title = "Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls",
abstract = "The Re mutant of Salmonella minnesota adheres in much greater numbers than the wild type to endothelial cells derived from the bovine pulmonary artery. Since the Re mutant is distinguished from wild-type S. minnesota by its ability to bind C1q and since endothelial cells possess receptors for C1q, we examined the role of C1q in the phagocytosis of the S. minnesota Re mutant. First, preincubating endothelial cells with C1q-enriched medium resulted in increased adherence of the Re mutant (17.9 x 104 versus 6.6 x 104). Second, preincubating the Re mutant with C1q-enriched medium resulted in increased numbers of adherent bacteria (62.1 x 104 versus 6.6 x 104). Preincubation of both endothelial cells and bacteria with C1q-enriched medium resulted in increased adherence above control levels but less adherence than when either cells or bacteria were preincubated separately in C1q-enriched medium. If serum depleted of C1q was used for preincubation of endothelial cells or bacteria, adherence was reduced below control levels. Thus, C1q plays an important role in the initial steps (recognition, binding, and ingestion) of phagocytosis. Next, the role of C1q was investigated in the respiratory burst response. Levels of superoxide anion released from endothelial cells 15 min after phagocytosis of the Re mutant (100 bacteria per endothelial cell) were assayed by measurement of the superoxide dismutase-inhibitable reduction of ferricytochrome c. Superoxide anion release was increased during phagocytosis of the Re mutant (35 nmol of O2- per 3 x 106 endothelial cells) and was also elevated above control values by incubation with soluble C1q (10 nmol of O2- per 3 x 106 endothelial cells). These results indicate a role for C1q in both the ingestion and the response of endothelial cells to the S. minnesota Re mutant.",
author = "Ryan, {U. S.} and Schultz, {D. R.} and Goodwin, {J. D.} and Vann, {J. M.} and Selvaraj, {M. P.} and Hart, {M. A.}",
year = "1989",
month = "1",
day = "1",
language = "English",
volume = "57",
pages = "1356--1362",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Role of C1q in phagocytosis of Salmonella minnesota by pulmonary endothelial cellls

AU - Ryan, U. S.

AU - Schultz, D. R.

AU - Goodwin, J. D.

AU - Vann, J. M.

AU - Selvaraj, M. P.

AU - Hart, M. A.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The Re mutant of Salmonella minnesota adheres in much greater numbers than the wild type to endothelial cells derived from the bovine pulmonary artery. Since the Re mutant is distinguished from wild-type S. minnesota by its ability to bind C1q and since endothelial cells possess receptors for C1q, we examined the role of C1q in the phagocytosis of the S. minnesota Re mutant. First, preincubating endothelial cells with C1q-enriched medium resulted in increased adherence of the Re mutant (17.9 x 104 versus 6.6 x 104). Second, preincubating the Re mutant with C1q-enriched medium resulted in increased numbers of adherent bacteria (62.1 x 104 versus 6.6 x 104). Preincubation of both endothelial cells and bacteria with C1q-enriched medium resulted in increased adherence above control levels but less adherence than when either cells or bacteria were preincubated separately in C1q-enriched medium. If serum depleted of C1q was used for preincubation of endothelial cells or bacteria, adherence was reduced below control levels. Thus, C1q plays an important role in the initial steps (recognition, binding, and ingestion) of phagocytosis. Next, the role of C1q was investigated in the respiratory burst response. Levels of superoxide anion released from endothelial cells 15 min after phagocytosis of the Re mutant (100 bacteria per endothelial cell) were assayed by measurement of the superoxide dismutase-inhibitable reduction of ferricytochrome c. Superoxide anion release was increased during phagocytosis of the Re mutant (35 nmol of O2- per 3 x 106 endothelial cells) and was also elevated above control values by incubation with soluble C1q (10 nmol of O2- per 3 x 106 endothelial cells). These results indicate a role for C1q in both the ingestion and the response of endothelial cells to the S. minnesota Re mutant.

AB - The Re mutant of Salmonella minnesota adheres in much greater numbers than the wild type to endothelial cells derived from the bovine pulmonary artery. Since the Re mutant is distinguished from wild-type S. minnesota by its ability to bind C1q and since endothelial cells possess receptors for C1q, we examined the role of C1q in the phagocytosis of the S. minnesota Re mutant. First, preincubating endothelial cells with C1q-enriched medium resulted in increased adherence of the Re mutant (17.9 x 104 versus 6.6 x 104). Second, preincubating the Re mutant with C1q-enriched medium resulted in increased numbers of adherent bacteria (62.1 x 104 versus 6.6 x 104). Preincubation of both endothelial cells and bacteria with C1q-enriched medium resulted in increased adherence above control levels but less adherence than when either cells or bacteria were preincubated separately in C1q-enriched medium. If serum depleted of C1q was used for preincubation of endothelial cells or bacteria, adherence was reduced below control levels. Thus, C1q plays an important role in the initial steps (recognition, binding, and ingestion) of phagocytosis. Next, the role of C1q was investigated in the respiratory burst response. Levels of superoxide anion released from endothelial cells 15 min after phagocytosis of the Re mutant (100 bacteria per endothelial cell) were assayed by measurement of the superoxide dismutase-inhibitable reduction of ferricytochrome c. Superoxide anion release was increased during phagocytosis of the Re mutant (35 nmol of O2- per 3 x 106 endothelial cells) and was also elevated above control values by incubation with soluble C1q (10 nmol of O2- per 3 x 106 endothelial cells). These results indicate a role for C1q in both the ingestion and the response of endothelial cells to the S. minnesota Re mutant.

UR - http://www.scopus.com/inward/record.url?scp=0024524272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024524272&partnerID=8YFLogxK

M3 - Article

C2 - 2540090

AN - SCOPUS:0024524272

VL - 57

SP - 1356

EP - 1362

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -