Role of an estrogen receptor-dependent mechanism in the regulation of estrogen receptor mRNA in MCF-7 cells

M. Saceda, Marc E Lippman, R. K. Lindsey, M. Puente, M. B. Martin

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

We have previously demonstrated that regulation of estrogen receptor (ER) expression in MCF-7 breast cancer cells in a complex process involving transcriptional and posttranscriptional regulation by estradiol. Treatment of MCF-7 cells with estradiol results in the down-regulation of receptor expression; posttranslational suppression of receptor mRNA appears to be the predominant mechanism. To determine whether posttranscriptional regulation of ER gene expression is mediated by an ER-dependent mechanism independent of protein synthesis, we have used the competitive estrogen antagonist, 4-hydroxytamoxifen, and the inhibitor of protein synthesis, cycloheximide, to study regulation of ER mRNA by estradiol. 4-Hydroxytamoxifen had no effect on the steady-state level of receptor mRNA and effectively blocked the suppression of ER mRNA by estradiol. The metabolic inhibitor, cycloheximide, was unable to prevent the estrogen induced decrease in ER mRNA. These data provide evidence that the posttranscriptional suppression of ER expression through estradiol is mediated through the ER independent of protein synthesis. A study of the effects of estradiol on the steady-state levels of nuclear and cytoplasmic receptor mRNA suggest that posttranscriptional suppression is a nuclear event.

Original languageEnglish
Pages (from-to)1782-1787
Number of pages6
JournalMolecular Endocrinology
Volume3
Issue number11
StatePublished - Dec 15 1989
Externally publishedYes

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MCF-7 Cells
Estrogen Receptors
Estradiol
Messenger RNA
Cycloheximide
Estrogen Antagonists
Protein Synthesis Inhibitors
Cytoplasmic and Nuclear Receptors
Estrogens
Down-Regulation
Breast Neoplasms
Gene Expression
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Saceda, M., Lippman, M. E., Lindsey, R. K., Puente, M., & Martin, M. B. (1989). Role of an estrogen receptor-dependent mechanism in the regulation of estrogen receptor mRNA in MCF-7 cells. Molecular Endocrinology, 3(11), 1782-1787.

Role of an estrogen receptor-dependent mechanism in the regulation of estrogen receptor mRNA in MCF-7 cells. / Saceda, M.; Lippman, Marc E; Lindsey, R. K.; Puente, M.; Martin, M. B.

In: Molecular Endocrinology, Vol. 3, No. 11, 15.12.1989, p. 1782-1787.

Research output: Contribution to journalArticle

Saceda, M, Lippman, ME, Lindsey, RK, Puente, M & Martin, MB 1989, 'Role of an estrogen receptor-dependent mechanism in the regulation of estrogen receptor mRNA in MCF-7 cells', Molecular Endocrinology, vol. 3, no. 11, pp. 1782-1787.
Saceda M, Lippman ME, Lindsey RK, Puente M, Martin MB. Role of an estrogen receptor-dependent mechanism in the regulation of estrogen receptor mRNA in MCF-7 cells. Molecular Endocrinology. 1989 Dec 15;3(11):1782-1787.
Saceda, M. ; Lippman, Marc E ; Lindsey, R. K. ; Puente, M. ; Martin, M. B. / Role of an estrogen receptor-dependent mechanism in the regulation of estrogen receptor mRNA in MCF-7 cells. In: Molecular Endocrinology. 1989 ; Vol. 3, No. 11. pp. 1782-1787.
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