Robust single-cell DNA methylome profiling with snmC-seq2

Chongyuan Luo; Angeline Rivkin; Jingtian Zhou; Justin P. Sandoval; Laurie Kurihara; Jacinta Lucero; Rosa Castanon; Joseph R. Nery; António Pinto-Duarte; Brian Bui; Conor Fitzpatrick; Carolyn O’Connor; Seth Ruga; Marc E. Van Eden; David A. Davis; Deborah C. Mash; M. Margarita Behrens; Joseph R. Ecker

doi:10.1038/s41467-018-06355-2

Robust single-cell DNA methylome profiling with snmC-seq2

Chongyuan Luo, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, Joseph R. Nery, António Pinto-Duarte, Brian Bui, Conor Fitzpatrick, Carolyn O’Connor, Seth Ruga, Marc E. Van Eden, David A. Davis, Deborah C. Mash, M. Margarita Behrens, Joseph R. Ecker

Neurology

Research output: Contribution to journal › Article

14 Scopus citations

Abstract

Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies.

Original language	English (US)
Article number	3824
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-06355-2
State	Published - Dec 1 2018

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/s41467-018-06355-2

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Luo, C., Rivkin, A., Zhou, J., Sandoval, J. P., Kurihara, L., Lucero, J., Castanon, R., Nery, J. R., Pinto-Duarte, A., Bui, B., Fitzpatrick, C., O’Connor, C., Ruga, S., Van Eden, M. E., Davis, D. A., Mash, D. C., Behrens, M. M., & Ecker, J. R. (2018). Robust single-cell DNA methylome profiling with snmC-seq2. Nature communications, 9(1), [3824]. https://doi.org/10.1038/s41467-018-06355-2

Robust single-cell DNA methylome profiling with snmC-seq2. / Luo, Chongyuan; Rivkin, Angeline; Zhou, Jingtian; Sandoval, Justin P.; Kurihara, Laurie; Lucero, Jacinta; Castanon, Rosa; Nery, Joseph R.; Pinto-Duarte, António; Bui, Brian; Fitzpatrick, Conor; O’Connor, Carolyn; Ruga, Seth; Van Eden, Marc E.; Davis, David A.; Mash, Deborah C.; Behrens, M. Margarita; Ecker, Joseph R.

In: Nature communications, Vol. 9, No. 1, 3824, 01.12.2018.

Research output: Contribution to journal › Article

Luo, Chongyuan ; Rivkin, Angeline ; Zhou, Jingtian ; Sandoval, Justin P. ; Kurihara, Laurie ; Lucero, Jacinta ; Castanon, Rosa ; Nery, Joseph R. ; Pinto-Duarte, António ; Bui, Brian ; Fitzpatrick, Conor ; O’Connor, Carolyn ; Ruga, Seth ; Van Eden, Marc E. ; Davis, David A. ; Mash, Deborah C. ; Behrens, M. Margarita ; Ecker, Joseph R. / Robust single-cell DNA methylome profiling with snmC-seq2. In: Nature communications. 2018 ; Vol. 9, No. 1.

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