Robust CNS regeneration after complete spinal cord transection using aligned poly-l-lactic acid microfibers

Andres Hurtado, Jared M. Cregg, Han B. Wang, Dane F. Wendell, Martin Oudega, Ryan J. Gilbert, John W. McDonald

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Following spinal cord injury, axons fail to regenerate without exogenous intervention. In this study we report that aligned microfiber-based grafts foster robust regeneration of vascularized CNS tissue. Film, random, and aligned microfiber-based conduits were grafted into a 3 mm thoracic rat spinal cord gap created by complete transection. Over the course of 4 weeks, microtopography presented by aligned or random poly- l-lactic acid microfibers facilitated infiltration of host tissue, and the initial 3 mm gap was closed by endogenous cell populations. This bulk tissue response was composed of regenerating axons accompanied by morphologically aligned astrocytes. Aligned fibers promoted long distance (2055 ± 150 μm), rostrocaudal axonal regeneration, significantly greater than random fiber (1162 ± 87 μm) and film (413 ± 199 μm) controls. Retrograde tracing indicated that regenerating axons originated from propriospinal neurons of the rostral spinal cord, and supraspinal neurons of the reticular formation, red nucleus, raphe and vestibular nuclei. Our findings outline a form of regeneration within the central nervous system that holds important implications for regeneration biology.

Original languageEnglish (US)
Pages (from-to)6068-6079
Number of pages12
JournalBiomaterials
Volume32
Issue number26
DOIs
StatePublished - Sep 2011
Externally publishedYes

Fingerprint

Lactic acid
Spinal Cord Injuries
Regeneration
Lactic Acid
Tissue
Axons
Neurons
Spinal Cord
Fibers
Neurology
Red Nucleus
Vestibular Nuclei
Infiltration
Grafts
Raphe Nuclei
Reticular Formation
Rats
Cells
Astrocytes
Thorax

Keywords

  • Aligned microfibers
  • Axon guidance
  • Axonal regeneration
  • Electrospinning
  • Polylactic acid
  • Spinal cord injury

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Hurtado, A., Cregg, J. M., Wang, H. B., Wendell, D. F., Oudega, M., Gilbert, R. J., & McDonald, J. W. (2011). Robust CNS regeneration after complete spinal cord transection using aligned poly-l-lactic acid microfibers. Biomaterials, 32(26), 6068-6079. https://doi.org/10.1016/j.biomaterials.2011.05.006

Robust CNS regeneration after complete spinal cord transection using aligned poly-l-lactic acid microfibers. / Hurtado, Andres; Cregg, Jared M.; Wang, Han B.; Wendell, Dane F.; Oudega, Martin; Gilbert, Ryan J.; McDonald, John W.

In: Biomaterials, Vol. 32, No. 26, 09.2011, p. 6068-6079.

Research output: Contribution to journalArticle

Hurtado, Andres ; Cregg, Jared M. ; Wang, Han B. ; Wendell, Dane F. ; Oudega, Martin ; Gilbert, Ryan J. ; McDonald, John W. / Robust CNS regeneration after complete spinal cord transection using aligned poly-l-lactic acid microfibers. In: Biomaterials. 2011 ; Vol. 32, No. 26. pp. 6068-6079.
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