RNase T is responsible for the end-turnover of tRNA in Escherichia coli

Murray P Deutscher, C. W. Marlor, R. Zaniewski

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A mutant strain deficient in RNase T was isolated and used to study the role of this enzyme in Escherichia coli. Strains lacking as much as 70% of RNase T activity, alone or in combination with the absence of other RNases, display normal growth properties. However, in cca strains, which lack tRNA nucleotidyltransferase, RNase T-deficient derivatives accumulate lower levels of defective tRNA and grow at increased rates compared to their RNase T+ parents. Slow-growing cca strains revert to a faster-growing form that contains less defective tRNA but which is still cca. All of these strains have decreased levels of RNase T. These data indicate that RNase T is responsible for nucleotide removal during the tRNA end-turnover process and that the amount of defective tRNA in cells is determined by the relative levels of RNase T and tRNA nucleotidyltransferase.

Original languageEnglish
Pages (from-to)6427-6430
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number19
StatePublished - Dec 1 1985
Externally publishedYes

Fingerprint

Transfer RNA
Escherichia coli
Ribonucleases
exoribonuclease T
Nucleotides
Enzymes
Growth

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

RNase T is responsible for the end-turnover of tRNA in Escherichia coli. / Deutscher, Murray P; Marlor, C. W.; Zaniewski, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 19, 01.12.1985, p. 6427-6430.

Research output: Contribution to journalArticle

Deutscher, Murray P ; Marlor, C. W. ; Zaniewski, R. / RNase T is responsible for the end-turnover of tRNA in Escherichia coli. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 19. pp. 6427-6430.
@article{6ab372b65b7848538a8ed7514f033889,
title = "RNase T is responsible for the end-turnover of tRNA in Escherichia coli",
abstract = "A mutant strain deficient in RNase T was isolated and used to study the role of this enzyme in Escherichia coli. Strains lacking as much as 70{\%} of RNase T activity, alone or in combination with the absence of other RNases, display normal growth properties. However, in cca strains, which lack tRNA nucleotidyltransferase, RNase T-deficient derivatives accumulate lower levels of defective tRNA and grow at increased rates compared to their RNase T+ parents. Slow-growing cca strains revert to a faster-growing form that contains less defective tRNA but which is still cca. All of these strains have decreased levels of RNase T. These data indicate that RNase T is responsible for nucleotide removal during the tRNA end-turnover process and that the amount of defective tRNA in cells is determined by the relative levels of RNase T and tRNA nucleotidyltransferase.",
author = "Deutscher, {Murray P} and Marlor, {C. W.} and R. Zaniewski",
year = "1985",
month = "12",
day = "1",
language = "English",
volume = "82",
pages = "6427--6430",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "19",

}

TY - JOUR

T1 - RNase T is responsible for the end-turnover of tRNA in Escherichia coli

AU - Deutscher, Murray P

AU - Marlor, C. W.

AU - Zaniewski, R.

PY - 1985/12/1

Y1 - 1985/12/1

N2 - A mutant strain deficient in RNase T was isolated and used to study the role of this enzyme in Escherichia coli. Strains lacking as much as 70% of RNase T activity, alone or in combination with the absence of other RNases, display normal growth properties. However, in cca strains, which lack tRNA nucleotidyltransferase, RNase T-deficient derivatives accumulate lower levels of defective tRNA and grow at increased rates compared to their RNase T+ parents. Slow-growing cca strains revert to a faster-growing form that contains less defective tRNA but which is still cca. All of these strains have decreased levels of RNase T. These data indicate that RNase T is responsible for nucleotide removal during the tRNA end-turnover process and that the amount of defective tRNA in cells is determined by the relative levels of RNase T and tRNA nucleotidyltransferase.

AB - A mutant strain deficient in RNase T was isolated and used to study the role of this enzyme in Escherichia coli. Strains lacking as much as 70% of RNase T activity, alone or in combination with the absence of other RNases, display normal growth properties. However, in cca strains, which lack tRNA nucleotidyltransferase, RNase T-deficient derivatives accumulate lower levels of defective tRNA and grow at increased rates compared to their RNase T+ parents. Slow-growing cca strains revert to a faster-growing form that contains less defective tRNA but which is still cca. All of these strains have decreased levels of RNase T. These data indicate that RNase T is responsible for nucleotide removal during the tRNA end-turnover process and that the amount of defective tRNA in cells is determined by the relative levels of RNase T and tRNA nucleotidyltransferase.

UR - http://www.scopus.com/inward/record.url?scp=0022366980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022366980&partnerID=8YFLogxK

M3 - Article

C2 - 2413440

AN - SCOPUS:0022366980

VL - 82

SP - 6427

EP - 6430

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 19

ER -