RNase T is responsible for the end-turnover of tRNA in Escherichia coli

M. P. Deutscher, C. W. Marlor, R. Zaniewski

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


A mutant strain deficient in RNase T was isolated and used to study the role of this enzyme in Escherichia coli. Strains lacking as much as 70% of RNase T activity, alone or in combination with the absence of other RNases, display normal growth properties. However, in cca strains, which lack tRNA nucleotidyltransferase, RNase T-deficient derivatives accumulate lower levels of defective tRNA and grow at increased rates compared to their RNase T+ parents. Slow-growing cca strains revert to a faster-growing form that contains less defective tRNA but which is still cca. All of these strains have decreased levels of RNase T. These data indicate that RNase T is responsible for nucleotide removal during the tRNA end-turnover process and that the amount of defective tRNA in cells is determined by the relative levels of RNase T and tRNA nucleotidyltransferase.

Original languageEnglish (US)
Pages (from-to)6427-6430
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number19
StatePublished - 1985

ASJC Scopus subject areas

  • General


Dive into the research topics of 'RNase T is responsible for the end-turnover of tRNA in Escherichia coli'. Together they form a unique fingerprint.

Cite this