RNAi screen indicates widespread biological function for human natural antisense transcripts

Mohammad A Faghihi, Jannet Kocerha, Farzaneh Modarresi, Pär G. Engström, Alistair M. Chalk, Shaun P Brothers, Eric Koesema, Georges St. Laurent, Claes R Wahlestedt

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Natural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a lossof- function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.

Original languageEnglish
Article numbere13177
JournalPLoS One
Volume5
Issue number10
DOIs
StatePublished - Nov 4 2010
Externally publishedYes

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messenger RNA
small interfering RNA
RNA Interference
RNA interference
cell viability
RNA
phenotype
genome
Small Interfering RNA
Cell Survival
Genome
Phenotype
Genes
Cells
Modulation
Molecules

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

RNAi screen indicates widespread biological function for human natural antisense transcripts. / Faghihi, Mohammad A; Kocerha, Jannet; Modarresi, Farzaneh; Engström, Pär G.; Chalk, Alistair M.; Brothers, Shaun P; Koesema, Eric; St. Laurent, Georges; Wahlestedt, Claes R.

In: PLoS One, Vol. 5, No. 10, e13177, 04.11.2010.

Research output: Contribution to journalArticle

Faghihi, Mohammad A ; Kocerha, Jannet ; Modarresi, Farzaneh ; Engström, Pär G. ; Chalk, Alistair M. ; Brothers, Shaun P ; Koesema, Eric ; St. Laurent, Georges ; Wahlestedt, Claes R. / RNAi screen indicates widespread biological function for human natural antisense transcripts. In: PLoS One. 2010 ; Vol. 5, No. 10.
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