RNAi screen indicates widespread biological function for human natural antisense transcripts

Mohammad Ali Faghihi, Jannet Kocerha, Farzaneh Modarresi, Pär G. Engström, Alistair M. Chalk, Shaun P. Brothers, Eric Koesema, Georges St. Laurent, Claes Wahlestedt

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Natural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a lossof- function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.

Original languageEnglish (US)
Article numbere13177
JournalPloS one
Volume5
Issue number10
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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