RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer - TRBP complex

Yukio Kawahara, Boris Zinshteyn, Thimmaiah P. Chendrimada, Ramin Shiekhattar, Kazuko Nishikura

Research output: Contribution to journalArticle

267 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) mediate translational repression or degradation of their target messenger RNAs by RNA interference (RNAi). The primary transcripts of miRNA genes (pri-miRNAs) are sequentially processed by the nuclear Drosha - DGCR8 complex to approximately 60-70 nucleotide (nt) intermediates (pre-miRNAs) and then by the cytoplasmic Dicer - TRBP complex to approximately 20-22 nt mature miRNAs. Certain pri-miRNAs are subject to RNA editing that converts adenosine to inosine (A → I RNA editing); however, the fate of edited pri-miRNAs is mostly unknown. Here, we provide evidence that RNA editing of pri-miR-151 results in complete blockage of its cleavage by Dicer and accumulation of edited pre-miR-151 RNAs. Our results indicate that A → I conversion at two specific positions of the pre-miRNA foldback structure can affect its interaction with the Dicer - TRBP complex, showing a new regulatory role of A → I RNA editing in miRNA biogenesis.

Original languageEnglish (US)
Pages (from-to)763-769
Number of pages7
JournalEMBO Reports
Volume8
Issue number8
DOIs
StatePublished - Aug 2007
Externally publishedYes

Fingerprint

RNA Editing
MicroRNAs
RNA
Genes
Nucleotides
Inosine
Pseudogenes
RNA Interference
Adenosine
Degradation
Messenger RNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer - TRBP complex. / Kawahara, Yukio; Zinshteyn, Boris; Chendrimada, Thimmaiah P.; Shiekhattar, Ramin; Nishikura, Kazuko.

In: EMBO Reports, Vol. 8, No. 8, 08.2007, p. 763-769.

Research output: Contribution to journalArticle

Kawahara, Yukio ; Zinshteyn, Boris ; Chendrimada, Thimmaiah P. ; Shiekhattar, Ramin ; Nishikura, Kazuko. / RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer - TRBP complex. In: EMBO Reports. 2007 ; Vol. 8, No. 8. pp. 763-769.
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