Abstract
MicroRNAs (miRNAs) mediate translational repression or degradation of their target messenger RNAs by RNA interference (RNAi). The primary transcripts of miRNA genes (pri-miRNAs) are sequentially processed by the nuclear Drosha - DGCR8 complex to approximately 60-70 nucleotide (nt) intermediates (pre-miRNAs) and then by the cytoplasmic Dicer - TRBP complex to approximately 20-22 nt mature miRNAs. Certain pri-miRNAs are subject to RNA editing that converts adenosine to inosine (A → I RNA editing); however, the fate of edited pri-miRNAs is mostly unknown. Here, we provide evidence that RNA editing of pri-miR-151 results in complete blockage of its cleavage by Dicer and accumulation of edited pre-miR-151 RNAs. Our results indicate that A → I conversion at two specific positions of the pre-miRNA foldback structure can affect its interaction with the Dicer - TRBP complex, showing a new regulatory role of A → I RNA editing in miRNA biogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 763-769 |
| Number of pages | 7 |
| Journal | EMBO Reports |
| Volume | 8 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Cell Biology
Cite this
RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer - TRBP complex. / Kawahara, Yukio; Zinshteyn, Boris; Chendrimada, Thimmaiah P.; Shiekhattar, Ramin; Nishikura, Kazuko.
In: EMBO Reports, Vol. 8, No. 8, 08.2007, p. 763-769.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer - TRBP complex
AU - Kawahara, Yukio
AU - Zinshteyn, Boris
AU - Chendrimada, Thimmaiah P.
AU - Shiekhattar, Ramin
AU - Nishikura, Kazuko
PY - 2007/8
Y1 - 2007/8
N2 - MicroRNAs (miRNAs) mediate translational repression or degradation of their target messenger RNAs by RNA interference (RNAi). The primary transcripts of miRNA genes (pri-miRNAs) are sequentially processed by the nuclear Drosha - DGCR8 complex to approximately 60-70 nucleotide (nt) intermediates (pre-miRNAs) and then by the cytoplasmic Dicer - TRBP complex to approximately 20-22 nt mature miRNAs. Certain pri-miRNAs are subject to RNA editing that converts adenosine to inosine (A → I RNA editing); however, the fate of edited pri-miRNAs is mostly unknown. Here, we provide evidence that RNA editing of pri-miR-151 results in complete blockage of its cleavage by Dicer and accumulation of edited pre-miR-151 RNAs. Our results indicate that A → I conversion at two specific positions of the pre-miRNA foldback structure can affect its interaction with the Dicer - TRBP complex, showing a new regulatory role of A → I RNA editing in miRNA biogenesis.
AB - MicroRNAs (miRNAs) mediate translational repression or degradation of their target messenger RNAs by RNA interference (RNAi). The primary transcripts of miRNA genes (pri-miRNAs) are sequentially processed by the nuclear Drosha - DGCR8 complex to approximately 60-70 nucleotide (nt) intermediates (pre-miRNAs) and then by the cytoplasmic Dicer - TRBP complex to approximately 20-22 nt mature miRNAs. Certain pri-miRNAs are subject to RNA editing that converts adenosine to inosine (A → I RNA editing); however, the fate of edited pri-miRNAs is mostly unknown. Here, we provide evidence that RNA editing of pri-miR-151 results in complete blockage of its cleavage by Dicer and accumulation of edited pre-miR-151 RNAs. Our results indicate that A → I conversion at two specific positions of the pre-miRNA foldback structure can affect its interaction with the Dicer - TRBP complex, showing a new regulatory role of A → I RNA editing in miRNA biogenesis.
UR - http://www.scopus.com/inward/record.url?scp=34547629436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547629436&partnerID=8YFLogxK
U2 - 10.1038/sj.embor.7401011
DO - 10.1038/sj.embor.7401011
M3 - Article
C2 - 17599088
AN - SCOPUS:34547629436
VL - 8
SP - 763
EP - 769
JO - EMBO Reports
JF - EMBO Reports
SN - 1469-221X
IS - 8
ER -