Abstract
MicroRNAs (miRNAs) mediate translational repression or degradation of their target messenger RNAs by RNA interference (RNAi). The primary transcripts of miRNA genes (pri-miRNAs) are sequentially processed by the nuclear Drosha - DGCR8 complex to approximately 60-70 nucleotide (nt) intermediates (pre-miRNAs) and then by the cytoplasmic Dicer - TRBP complex to approximately 20-22 nt mature miRNAs. Certain pri-miRNAs are subject to RNA editing that converts adenosine to inosine (A → I RNA editing); however, the fate of edited pri-miRNAs is mostly unknown. Here, we provide evidence that RNA editing of pri-miR-151 results in complete blockage of its cleavage by Dicer and accumulation of edited pre-miR-151 RNAs. Our results indicate that A → I conversion at two specific positions of the pre-miRNA foldback structure can affect its interaction with the Dicer - TRBP complex, showing a new regulatory role of A → I RNA editing in miRNA biogenesis.
Original language | English (US) |
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Pages (from-to) | 763-769 |
Number of pages | 7 |
Journal | EMBO Reports |
Volume | 8 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2007 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics