RNA-binding protein LIN28 is a marker for primary extragonadal germ cell tumors: An immunohistochemical study of 131 cases

Dengfeng Cao, Aijun Liu, Fenghua Wang, Robert W. Allan, Kaiyong Mei, Yan Peng, Jun Du, Shuangping Guo, Ty W. Abel, Zhaoli Lane, Joe Ma, Maria Rodriguez, Shirin Akhi, Neha Dehiya, Jianping Li

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Abstract

LIN28 has been shown to have an important role in primordial germ cell development and malignant transformation of germ cells in mouse. In this study, we examined the immunohistochemical profile of LIN28 in 131 primary human extragonadal germ cell tumors (central nervous system (CNS) 76, mediastinum 17, sacrococcygeal region 30, pelvis 3, vagina 2, liver 1, omentum 1, and retroperitoneum 1), including the following tumors and/or components: 57 seminomas/germinomas, 10 embryonal carcinomas, 74 yolk sac tumors, 6 choriocarcinomas, 15 mature, and 13 immature teratomas. We compared LIN28 with SALL4 to assess its diagnostic value. To determine its specificity, we examined LIN28 in 406 extragonadal-non-germ cell tumors (103 carcinomas, 91 sarcomas, 9 melanomas, 12 mesotheliomas, 83 lymphomas, 9 plasmacytomas, 82 CNS tumors, and 17 thymic epithelial tumors). The staining was semi-quantitatively scored as 0 (no cell stained), 1 (0-30%), 2 (31-60%), 3 (61-90%), and 4 (>90%). LIN28 staining was seen in all seminomas/germinomas (3 in 1 and 4 in 56), embryonal carcinomas (4 in all 10), and yolk sac tumors (3 in 3 and 4 in 71). Variable LIN28 staining was seen in 5 of 6 choriocarcinomas (1 to 4), 8 of 13 immature teratomas (1 to 2 in immature elements), and in 1 of 15 mature teratomas (1). Only 11 of 406 non-germ cell tumors showed 1 LIN28 staining. Therefore, LIN28 is a sensitive (100% sensitivity) marker for primary extragonadal seminomas/germinomas, embryonal carcinomas, and yolk sac tumors with high specificity. Compared with SALL4, LIN28 demonstrated a similar level of diagnostic sensitivity for seminomas/germinomas and embryonal carcinomas. For primary extragonadal yolk sac tumors, although SALL4 stained all tumors (1 in 1, 2 in 2, 3 in 10, and 4 in 61), LIN28 stained more tumor cells (mean 95 vs 90%, P0.03) and was therefore more sensitive. For primary extragonadal yolk sac tumors, combining LIN28 and SALL4 can achieve a higher diagnostic sensitivity than either alone.

Original languageEnglish
Pages (from-to)288-296
Number of pages9
JournalModern Pathology
Volume24
Issue number2
DOIs
StatePublished - Feb 1 2011

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Endodermal Sinus Tumor
RNA-Binding Proteins
Germ Cell and Embryonal Neoplasms
Germinoma
Embryonal Carcinoma
Seminoma
Teratoma
Staining and Labeling
Choriocarcinoma
Neoplasms
Germ Cells
Sacrococcygeal Region
Nervous System Neoplasms
Central Nervous System Neoplasms
Omentum
Plasmacytoma
Mesothelioma
Mediastinum
Vagina
Pelvis

Keywords

  • embryonal carcinoma
  • germinomas
  • LIN28
  • primary extragonadal germ cell tumor
  • seminoma
  • yolk sac tumor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

RNA-binding protein LIN28 is a marker for primary extragonadal germ cell tumors : An immunohistochemical study of 131 cases. / Cao, Dengfeng; Liu, Aijun; Wang, Fenghua; Allan, Robert W.; Mei, Kaiyong; Peng, Yan; Du, Jun; Guo, Shuangping; Abel, Ty W.; Lane, Zhaoli; Ma, Joe; Rodriguez, Maria; Akhi, Shirin; Dehiya, Neha; Li, Jianping.

In: Modern Pathology, Vol. 24, No. 2, 01.02.2011, p. 288-296.

Research output: Contribution to journalArticle

Cao, D, Liu, A, Wang, F, Allan, RW, Mei, K, Peng, Y, Du, J, Guo, S, Abel, TW, Lane, Z, Ma, J, Rodriguez, M, Akhi, S, Dehiya, N & Li, J 2011, 'RNA-binding protein LIN28 is a marker for primary extragonadal germ cell tumors: An immunohistochemical study of 131 cases', Modern Pathology, vol. 24, no. 2, pp. 288-296. https://doi.org/10.1038/modpathol.2010.195
Cao, Dengfeng ; Liu, Aijun ; Wang, Fenghua ; Allan, Robert W. ; Mei, Kaiyong ; Peng, Yan ; Du, Jun ; Guo, Shuangping ; Abel, Ty W. ; Lane, Zhaoli ; Ma, Joe ; Rodriguez, Maria ; Akhi, Shirin ; Dehiya, Neha ; Li, Jianping. / RNA-binding protein LIN28 is a marker for primary extragonadal germ cell tumors : An immunohistochemical study of 131 cases. In: Modern Pathology. 2011 ; Vol. 24, No. 2. pp. 288-296.
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AU - Cao, Dengfeng

AU - Liu, Aijun

AU - Wang, Fenghua

AU - Allan, Robert W.

AU - Mei, Kaiyong

AU - Peng, Yan

AU - Du, Jun

AU - Guo, Shuangping

AU - Abel, Ty W.

AU - Lane, Zhaoli

AU - Ma, Joe

AU - Rodriguez, Maria

AU - Akhi, Shirin

AU - Dehiya, Neha

AU - Li, Jianping

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N2 - LIN28 has been shown to have an important role in primordial germ cell development and malignant transformation of germ cells in mouse. In this study, we examined the immunohistochemical profile of LIN28 in 131 primary human extragonadal germ cell tumors (central nervous system (CNS) 76, mediastinum 17, sacrococcygeal region 30, pelvis 3, vagina 2, liver 1, omentum 1, and retroperitoneum 1), including the following tumors and/or components: 57 seminomas/germinomas, 10 embryonal carcinomas, 74 yolk sac tumors, 6 choriocarcinomas, 15 mature, and 13 immature teratomas. We compared LIN28 with SALL4 to assess its diagnostic value. To determine its specificity, we examined LIN28 in 406 extragonadal-non-germ cell tumors (103 carcinomas, 91 sarcomas, 9 melanomas, 12 mesotheliomas, 83 lymphomas, 9 plasmacytomas, 82 CNS tumors, and 17 thymic epithelial tumors). The staining was semi-quantitatively scored as 0 (no cell stained), 1 (0-30%), 2 (31-60%), 3 (61-90%), and 4 (>90%). LIN28 staining was seen in all seminomas/germinomas (3 in 1 and 4 in 56), embryonal carcinomas (4 in all 10), and yolk sac tumors (3 in 3 and 4 in 71). Variable LIN28 staining was seen in 5 of 6 choriocarcinomas (1 to 4), 8 of 13 immature teratomas (1 to 2 in immature elements), and in 1 of 15 mature teratomas (1). Only 11 of 406 non-germ cell tumors showed 1 LIN28 staining. Therefore, LIN28 is a sensitive (100% sensitivity) marker for primary extragonadal seminomas/germinomas, embryonal carcinomas, and yolk sac tumors with high specificity. Compared with SALL4, LIN28 demonstrated a similar level of diagnostic sensitivity for seminomas/germinomas and embryonal carcinomas. For primary extragonadal yolk sac tumors, although SALL4 stained all tumors (1 in 1, 2 in 2, 3 in 10, and 4 in 61), LIN28 stained more tumor cells (mean 95 vs 90%, P0.03) and was therefore more sensitive. For primary extragonadal yolk sac tumors, combining LIN28 and SALL4 can achieve a higher diagnostic sensitivity than either alone.

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