Right ventricular hypertrophy with early dysfunction: A proteomics study in a neonatal model

Amir M. Sheikh, Cindy Barrett, Nestor Villamizar, Oscar Alzate, Anne Marie Valente, J. Rene Herlong, Damian Craig, Andrew Lodge, Jeffrey Lawson, Carmelo Milano, James Jaggers

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Objective: Right ventricular hypertrophy and subsequent dysfunction is common in patients with congenital heart defects, but the molecular mechanisms underlying change from adaptive hypertrophy to dysfunction remain elusive. We used the novel technique of proteomics to characterize protein changes in right ventricular myocardium in a neonatal model of right ventricular hypertrophy and early dysfunction. Methods: Twelve neonatal piglets were equally randomized to pulmonary artery banding (PAB group), or sham operation (thoracotomy without banding). After 4 weeks, right ventricular morphology and function were assessed in vivo using magnetic resonance imaging. Animals were humanely killed. Proteomics of right ventricular myocardium was performed. Purified right ventricular proteins were separated by 2-dimensional difference gel electrophoresis using fluorescent cyanine dyes. After gel imaging, software analysis revealed protein spots differentially expressed between the 2 groups; these spots were excised and identified by mass spectrometry. Results: On magnetic resonance imaging, animals with pulmonary artery banding demonstrated significant right ventricular hypertrophy, cavity dilatation, and mild systolic impairment (right ventricular ejection fraction 39.8% ± 15% vs 56.7% ± 10% controls; P < .05). Right ventricular free wall mass on harvest confirmed right ventricular hypertrophy. Proteomic analysis revealed 18 proteins that were significantly differentially expressed: 5 structural proteins, 6 metabolic enzymes, 2 stress proteins, and 5 miscellaneous proteins. Expression of calsarcin-1 and vinculin was increased, as were certain metabolic enzymes, although F1-ATPase β-chain and heat shock protein 70 decreased. Conclusions: This is the first study characterizing right ventricular protein changes in a large animal model specifically capturing the change from compensated to maladaptive hypertrophy. These findings can guide future work at elucidating the mechanisms in the pathophysiology of neonatal right ventricular hypertrophy and dysfunction.

Original languageEnglish (US)
Pages (from-to)1146-1153
Number of pages8
JournalJournal of Thoracic and Cardiovascular Surgery
Issue number5
StatePublished - May 2009
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine


Dive into the research topics of 'Right ventricular hypertrophy with early dysfunction: A proteomics study in a neonatal model'. Together they form a unique fingerprint.

Cite this