Ribonuclease T: New exoribonuclease possibly involved in end-turnover of tRNA

Murray P Deutscher, C. W. Marlor, R. Zaniewski

Research output: Contribution to journalArticle

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Abstract

Examination of double mutants lacking one of the exoribonucleases, RNase II, RNase D, RNase BN, or RNase R, and also devoid of tRNA nucleotidyltransferase has suggested that none of these RNases participates in the end-turnover of tRNA. This prompted a search for and identification of a new exoribonuclease, termed TNase T. RNase T could be detected in mutant Escherichia coli strains lacking as many as three of the known exoribonucleases, and it could be separated from each of the four previously described RNases. RNase T is optimally active at pH 8-9 and requires a divalent cation for activity. The enzyme is sensitive to ionic strengths > 50 mM and is rapidly inactivated by heating at 45° C. Its preferred substrate is tRNA-C-C-[14C]A, with much less activity shown against tRNA-C-C. RNase T is an exoribonuclease that initiates attack at the 3' hydroxyl terminus of tRNA and releases AMP in a random mode of hydrolysis. The possible involvement of RNase T in end-turnover of tRNA and in RNA metabolism in general are discussed.

Original languageEnglish
Pages (from-to)4290-4293
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number14 I
StatePublished - Jan 1 1984
Externally publishedYes

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Exoribonucleases
Transfer RNA
Ribonucleases
Ribonuclease III
Micrococcal Nuclease
Divalent Cations
Adenosine Monophosphate
Hydroxyl Radical
Osmolar Concentration
Heating
Hydrolysis
exoribonuclease T
RNA
Escherichia coli
Enzymes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Ribonuclease T : New exoribonuclease possibly involved in end-turnover of tRNA. / Deutscher, Murray P; Marlor, C. W.; Zaniewski, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 14 I, 01.01.1984, p. 4290-4293.

Research output: Contribution to journalArticle

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