Ribonuclease D is not essential for the normal growth of Escherichia coli or bacteriophage T4 for the biosynthesis of a T4 suppressor tRNA

R. T. Blouin, R. Zaniewski, Murray P Deutscher

Research output: Contribution to journalArticle

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Abstract

Transposon Tn10-mediated rearrangement was used to isolate a strain of Escherichia coli carrying a deletion in the rnd region which is known to encode the structural gene for the putative 3' tRNA processing nuclease, RNase D. Genetic analysis indicated that about 0.4-0.5 min of the chromosome in the 39.5-40.0 min region was deleted. The mutant strain was devoid of RNase D activity, but other RNase activities were unaffected. The viability of the mutant strain and its normal growth characteristics indicate that RNase D is not essential for E. coli survival. The normal plating efficiency in this mutant host of wild type T4 and a T4 psu1+-amber double mutant indicates that RNase D is also not required for T4 growth or psu1+-tRNA processing. The implications of these findings for the role of RNase D in bacterial and bacteriophage tRNA metabolism, and the possible involvement of alternative enzymes, are discussed.

Original languageEnglish
Pages (from-to)1423-1426
Number of pages4
JournalJournal of Biological Chemistry
Volume258
Issue number3
StatePublished - Jan 1 1983
Externally publishedYes

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Ribonuclease III
Bacteriophage T4
Bacteriophages
Biosynthesis
Transfer RNA
Escherichia coli
Growth
Amber
Ribonucleases
Chromosomes
Processing
Plating
Metabolism
Genes
Enzymes

ASJC Scopus subject areas

  • Biochemistry

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Ribonuclease D is not essential for the normal growth of Escherichia coli or bacteriophage T4 for the biosynthesis of a T4 suppressor tRNA. / Blouin, R. T.; Zaniewski, R.; Deutscher, Murray P.

In: Journal of Biological Chemistry, Vol. 258, No. 3, 01.01.1983, p. 1423-1426.

Research output: Contribution to journalArticle

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