Rhenium-labeled somatostatin analog RC-160. 1H NMR and computer modeling conformational analysis

J. M. Varnum, M. L. Thakur, A. V. Schally, S. Jansen, K. H. Mayo

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The solution conformations of RC-160, cyclic n-Phe1-Cys2-Tyr3-D-Trp4-Lys 5-Val6-Cys7-Trp8-NH2, an analog of the tumor antiproliferatory neuropeptide somatostatin, and RC-160 labeled with rhenium (Re-RC-160), have been determined by using two-dimensional 1H NMR spectroscopy (600 MHz) and restrained molecular dynamics simulations. Re-RC-160 yields the same average solution conformation as does the apo form with an antiparallel β-sheet fold and a type II′ β-turn centered at D-Trp4-Lys5. These results indicate that the spatial topography of the side chains essential for somatostatin receptor binding is maintained in Re-RC-160.

Original languageEnglish (US)
Pages (from-to)12583-12588
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number17
StatePublished - Jul 7 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Rhenium-labeled somatostatin analog RC-160. <sup>1</sup>H NMR and computer modeling conformational analysis'. Together they form a unique fingerprint.

  • Cite this