Rh proteins and NH4+-activated NH4+-ATPase in the Magadi tilapia (Alcolapia grahami), a 100% ureotelic teleost fish

Chris M. Wood, C. Michele Nawata, Jonathan M. Wilson, Pierre Laurent, Claudine Chevalier, Harold L. Bergman, Adalto Bianchini, John N. Maina, Ora E. Johannsson, Lucas F. Bianchini, Geraldine D. Kavembe, Michael B. Papah, Rodi O. Ojoo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The small cichlid fish Alcolapia grahami lives in Lake Magadi, Kenya, one of the most extreme aquatic environments on Earth (pH ̃10, carbonate alkalinity ̃300?mequiv?l-1). The Magadi tilapia is the only 100% ureotelic teleost; it normally excretes no ammonia. This is interpreted as an evolutionary adaptation to overcome the near impossibility of sustaining an NH3 diffusion gradient across the gills against the high external pH. In standard ammoniotelic teleosts, branchial ammonia excretion is facilitated by Rh glycoproteins, and cortisol plays a role in upregulating these carriers, together with other components of a transport metabolon, so as to actively excrete ammonia during high environmental ammonia (HEA) exposure. In Magadi tilapia, we show that at least three Rh proteins (Rhag, Rhbg and Rhcg2) are expressed at the mRNA level in various tissues, and are recognized in the gills by specific antibodies. During HEA exposure, plasma ammonia levels and urea excretion rates increase markedly, and mRNA expression for the branchial urea transporter mtUT is elevated. Plasma cortisol increases and branchial mRNAs for Rhbg, Rhcg2 and Na+,K+-ATPase are all upregulated. Enzymatic activity of the latter is activated preferentially by NH4+ (versus K +), suggesting it can function as an NH4+-transporter. Model calculations suggest that active ammonia excretion against the gradient may become possible through a combination of Rh protein and NH4 +-activated Na+-ATPase function.

Original languageEnglish
Pages (from-to)2998-3007
Number of pages10
JournalJournal of Experimental Biology
Volume216
Issue number16
DOIs
StatePublished - Aug 1 2013

Fingerprint

Tilapia (Cichlidae)
Tilapia
teleost
Ammonia
adenosinetriphosphatase
Adenosine Triphosphatases
Fishes
ammonia
protein
fish
excretion
Proteins
proteins
Environmental Exposure
Messenger RNA
urea
Hydrocortisone
cortisol
transporters
gills

Keywords

  • Alcolapia grahami
  • Ammonia transport
  • Gills
  • High alkalinity
  • High environmental ammonia
  • K-ATPase
  • Na
  • Urea transport

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

Wood, C. M., Nawata, C. M., Wilson, J. M., Laurent, P., Chevalier, C., Bergman, H. L., ... Ojoo, R. O. (2013). Rh proteins and NH4+-activated NH4+-ATPase in the Magadi tilapia (Alcolapia grahami), a 100% ureotelic teleost fish. Journal of Experimental Biology, 216(16), 2998-3007. https://doi.org/10.1242/jeb.078634

Rh proteins and NH4+-activated NH4+-ATPase in the Magadi tilapia (Alcolapia grahami), a 100% ureotelic teleost fish. / Wood, Chris M.; Nawata, C. Michele; Wilson, Jonathan M.; Laurent, Pierre; Chevalier, Claudine; Bergman, Harold L.; Bianchini, Adalto; Maina, John N.; Johannsson, Ora E.; Bianchini, Lucas F.; Kavembe, Geraldine D.; Papah, Michael B.; Ojoo, Rodi O.

In: Journal of Experimental Biology, Vol. 216, No. 16, 01.08.2013, p. 2998-3007.

Research output: Contribution to journalArticle

Wood, CM, Nawata, CM, Wilson, JM, Laurent, P, Chevalier, C, Bergman, HL, Bianchini, A, Maina, JN, Johannsson, OE, Bianchini, LF, Kavembe, GD, Papah, MB & Ojoo, RO 2013, 'Rh proteins and NH4+-activated NH4+-ATPase in the Magadi tilapia (Alcolapia grahami), a 100% ureotelic teleost fish', Journal of Experimental Biology, vol. 216, no. 16, pp. 2998-3007. https://doi.org/10.1242/jeb.078634
Wood, Chris M. ; Nawata, C. Michele ; Wilson, Jonathan M. ; Laurent, Pierre ; Chevalier, Claudine ; Bergman, Harold L. ; Bianchini, Adalto ; Maina, John N. ; Johannsson, Ora E. ; Bianchini, Lucas F. ; Kavembe, Geraldine D. ; Papah, Michael B. ; Ojoo, Rodi O. / Rh proteins and NH4+-activated NH4+-ATPase in the Magadi tilapia (Alcolapia grahami), a 100% ureotelic teleost fish. In: Journal of Experimental Biology. 2013 ; Vol. 216, No. 16. pp. 2998-3007.
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