TY - JOUR
T1 - RGS2
T2 - Regulation of expression and nuclear localization
AU - Song, Ling
AU - Zmijewski, Jaroslaw W.
AU - Jope, Richard S.
N1 - Funding Information:
This research was supported by NIH Grants MH38752 and NS37768. We thank Dr. D. R. Forsdyke for generously providing the RGS2 cDNA, and Dr. Janusz Tucholski, Dr. Mathieu Lesort, and Anna Zmijewski for their suggestions and help with various aspects of this project.
PY - 2001
Y1 - 2001
N2 - RGS2, a Regulators of G-protein Signaling family member, regulates signaling activities of G-proteins, and RGS2 itself is controlled in part by regulation of its expression. This investigation extended previous studies of the regulation of RGS2 expression by examining the effects of stress, differentiation, and signaling activities on RGS2 mRNA level in human neuroblastoma SH-SY5Y cells. Cell stress induced by heat shock rapidly and transiently increased RGS2 mRNA levels, whereas differentiation to a neuronal phenotype reduced basal RGS2 mRNA levels by 50%. RGS2 mRNA levels were increased in differentiated cells by heat shock, carbachol, and activation of protein kinase C. After transient transfection of GFP-tagged RGS2, a predominant nuclear localization was observed by confocal microscopy. Thus, RGS2 expression is regulated by stress and differentiation, as well as by second messenger signaling, and transfected GFP-RGS2 is predominantly nuclear.
AB - RGS2, a Regulators of G-protein Signaling family member, regulates signaling activities of G-proteins, and RGS2 itself is controlled in part by regulation of its expression. This investigation extended previous studies of the regulation of RGS2 expression by examining the effects of stress, differentiation, and signaling activities on RGS2 mRNA level in human neuroblastoma SH-SY5Y cells. Cell stress induced by heat shock rapidly and transiently increased RGS2 mRNA levels, whereas differentiation to a neuronal phenotype reduced basal RGS2 mRNA levels by 50%. RGS2 mRNA levels were increased in differentiated cells by heat shock, carbachol, and activation of protein kinase C. After transient transfection of GFP-tagged RGS2, a predominant nuclear localization was observed by confocal microscopy. Thus, RGS2 expression is regulated by stress and differentiation, as well as by second messenger signaling, and transfected GFP-RGS2 is predominantly nuclear.
KW - G-protein
KW - Heat shock
KW - Muscarinic receptors
KW - RGS2
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U2 - 10.1006/bbrc.2001.4742
DO - 10.1006/bbrc.2001.4742
M3 - Article
C2 - 11322774
AN - SCOPUS:0034789956
VL - 283
SP - 102
EP - 106
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -