RGS2: Regulation of expression and nuclear localization

Ling Song, Jaroslaw W. Zmijewski, Richard S. Jope

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

RGS2, a Regulators of G-protein Signaling family member, regulates signaling activities of G-proteins, and RGS2 itself is controlled in part by regulation of its expression. This investigation extended previous studies of the regulation of RGS2 expression by examining the effects of stress, differentiation, and signaling activities on RGS2 mRNA level in human neuroblastoma SH-SY5Y cells. Cell stress induced by heat shock rapidly and transiently increased RGS2 mRNA levels, whereas differentiation to a neuronal phenotype reduced basal RGS2 mRNA levels by 50%. RGS2 mRNA levels were increased in differentiated cells by heat shock, carbachol, and activation of protein kinase C. After transient transfection of GFP-tagged RGS2, a predominant nuclear localization was observed by confocal microscopy. Thus, RGS2 expression is regulated by stress and differentiation, as well as by second messenger signaling, and transfected GFP-RGS2 is predominantly nuclear.

Original languageEnglish (US)
Pages (from-to)102-106
Number of pages5
JournalBiochemical and biophysical research communications
Volume283
Issue number1
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • G-protein
  • Heat shock
  • Muscarinic receptors
  • RGS2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'RGS2: Regulation of expression and nuclear localization'. Together they form a unique fingerprint.

  • Cite this