RGD-grafted poly-L-lysine-graft-(polyethylene glycol) copolymers block non-specific protein adsorption while promoting cell adhesion

Stephanie VandeVondele, Janos Vörös, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

267 Scopus citations

Abstract

A novel class of surface-active copolymers is described, designed to protect surfaces from nonspecific protein adsorption while still inducing specific cell attachment and spreading. A graft copolymer was synthesized, containing poly-(L-lysine) (PLL) as the backbone and substrate binding and poly(ethylene glycol) (PEG) as protein adsorption-resistant pendant side chains. A fraction of the grafted PEG was pendantly functionalized by covalent conjugation to the peptide motif RGD to induce cell binding. The graft copolymer spontaneously adsorbs from dilute aqueous solution onto negatively charged surfaces. The performance of RGD-modified PLL-g-PEG copolymers was analyzed in protein adsorption and cell culture assays. These coatings efficiently blocked the adsorption of serum proteins to Nb2O5 and tissue culture polystyrene while specifically supporting attachment and spreading of human dermal fibroblasts. This surface functionalization technology is expected to be valuable in both the biomaterial and biosensor fields, because different signals can easily be combined, and sterilization and application are straightforward and cost-effective.

Original languageEnglish (US)
Pages (from-to)784-790
Number of pages7
JournalBiotechnology and Bioengineering
Volume82
Issue number7
DOIs
StatePublished - Jun 30 2003

Keywords

  • Adhesion peptide
  • Biomaterials
  • Cell adhesion
  • Protein adsorption

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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