RFP2, c13ORF1, and FAM10A4 are the most likely tumor suppressor gene candidates for B-cell chronic lymphocytic leukemia

W. J. Van Everdink, A. Baranova, C. Lummen, T. Tyazhelova, M. W G Looman, Dmitry V Ivanov, E. Verlind, A. Pestova, H. Faber, A. Y. Van Der Veen, N. Yankovsky, E. Vellenga, C. H C M Buys

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Occurrence of 13q14 deletions between D13S273 and D13S25 in B-cell chronic lymphocytic leukemia (B-CLL) suggests that the region contains a tumor suppressor gene. We constructed a PAC/cosmid contig largely corresponding to a 380-kb 13q14 YAC insert that we found deleted in a high proportion of B-CLL patients. We found seven genes by exon trapping, cDNA screening and analysis/cDNA extension of known expressed sequence tags. One appeared to originate from another region of 13q. Recent publications have focused on two of the genes that most likely do not have a tumor suppressor role. This study evaluates the remaining four genes in the region by mutation scanning and theoretical analysis of putative encoded products. No mutations suggestive of a pathogenic effect were found. The 13q14 deletions may be a consequence of an inherent instability of the region, an idea supported by our finding of a considerable proportion of AluY repeats. Deletion of putative enhancer sequences and/or genes in the region may result in an inactivation of tumor suppression by a haploinsufficiency mechanism. We conclude that RFP2, c13ORF1, and a chromosome 13-specific ST13-like gene, FAM10A4, are the most likely candidates for such a type of B-CLL TSG.

Original languageEnglish
Pages (from-to)48-57
Number of pages10
JournalCancer Genetics and Cytogenetics
Volume146
Issue number1
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

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B-Cell Chronic Lymphocytic Leukemia
Tumor Suppressor Genes
Genes
Complementary DNA
Haploinsufficiency
Chromosomes, Human, Pair 13
Cosmids
Mutation
Expressed Sequence Tags
Exons
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

RFP2, c13ORF1, and FAM10A4 are the most likely tumor suppressor gene candidates for B-cell chronic lymphocytic leukemia. / Van Everdink, W. J.; Baranova, A.; Lummen, C.; Tyazhelova, T.; Looman, M. W G; Ivanov, Dmitry V; Verlind, E.; Pestova, A.; Faber, H.; Van Der Veen, A. Y.; Yankovsky, N.; Vellenga, E.; Buys, C. H C M.

In: Cancer Genetics and Cytogenetics, Vol. 146, No. 1, 01.10.2003, p. 48-57.

Research output: Contribution to journalArticle

Van Everdink, WJ, Baranova, A, Lummen, C, Tyazhelova, T, Looman, MWG, Ivanov, DV, Verlind, E, Pestova, A, Faber, H, Van Der Veen, AY, Yankovsky, N, Vellenga, E & Buys, CHCM 2003, 'RFP2, c13ORF1, and FAM10A4 are the most likely tumor suppressor gene candidates for B-cell chronic lymphocytic leukemia', Cancer Genetics and Cytogenetics, vol. 146, no. 1, pp. 48-57. https://doi.org/10.1016/S0165-4608(03)00126-2
Van Everdink, W. J. ; Baranova, A. ; Lummen, C. ; Tyazhelova, T. ; Looman, M. W G ; Ivanov, Dmitry V ; Verlind, E. ; Pestova, A. ; Faber, H. ; Van Der Veen, A. Y. ; Yankovsky, N. ; Vellenga, E. ; Buys, C. H C M. / RFP2, c13ORF1, and FAM10A4 are the most likely tumor suppressor gene candidates for B-cell chronic lymphocytic leukemia. In: Cancer Genetics and Cytogenetics. 2003 ; Vol. 146, No. 1. pp. 48-57.
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AU - Tyazhelova, T.

AU - Looman, M. W G

AU - Ivanov, Dmitry V

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