Reversible disruption of cell-matrix and cell-cell interactions by overexpression of sialomucin complex

Masanobu Komatsu, Coralie A. Carothers Carraway, Nevis L. Fregien, Kermit L. Carraway

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Sialomucin complex (SMC) is a large, heterodimeric glycoprotein complex composed of mucin (ASGP-1) and transmembrane (ASGP-2) subunits and expressed abundantly on the cell surface of ascites 13762 rat mammary adenocarcinoma cells. We have isolated recombinant cDNAs containing different numbers of ASGP-1 mucin repeats, which can be expressed as protein products with variable lengths. To study the anti-adhesive effect of SMC, these cDNAs were transfected into human cancer cell lines. Using a tetracycline-responsive, inducible expression system, we demonstrated that the overexpression of SMC induces morphology changes, cell detachment, and cell-cell dissociation of transfected A375 human melanoma cells in culture. The transition between the adherent and suspension states of the cells is fully reversible and dependent on the SMC expression level. The anti-adhesion effect of SMC was further analyzed kinetically by measuring the cell adhesion of transfected A375 melanoma and MCF-7 breast cancer cell lines to fibronectin, laminin, and collagen IV, demonstrating that SMC disrupts integrin-mediated cell adhesion to extracellular matrix proteins. The degree of this anti-adhesion effect was dependent on the number of mucin repeats in the SMC molecule as well as the level of cell surface expression.

Original languageEnglish
Pages (from-to)33245-33254
Number of pages10
JournalJournal of Biological Chemistry
Volume272
Issue number52
DOIs
StatePublished - Dec 26 1997

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Sialomucins
Cell Communication
Mucin-4
Mucins
Cell adhesion
Cell Adhesion
Melanoma
Adhesion
Complementary DNA
Cells
Cell Line
Mucin-1
Extracellular Matrix Proteins
Laminin
Tetracycline
Fibronectins
Ascites
Integrins
Adhesives
Rats

ASJC Scopus subject areas

  • Biochemistry

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Reversible disruption of cell-matrix and cell-cell interactions by overexpression of sialomucin complex. / Komatsu, Masanobu; Carothers Carraway, Coralie A.; Fregien, Nevis L.; Carraway, Kermit L.

In: Journal of Biological Chemistry, Vol. 272, No. 52, 26.12.1997, p. 33245-33254.

Research output: Contribution to journalArticle

Komatsu, Masanobu ; Carothers Carraway, Coralie A. ; Fregien, Nevis L. ; Carraway, Kermit L. / Reversible disruption of cell-matrix and cell-cell interactions by overexpression of sialomucin complex. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 52. pp. 33245-33254.
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