Reversible, cooperative reactions of yeast vacuole docking

Youngsoo Jun, Naomi Thorngren, Vincent J. Starai, Rutilio A. Fratti, Kevin Collins, William Wickner

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Homotypic yeast vacuole fusion occurs in three stages: (i) priming reactions, which are independent of vacuole clustering, (ii) docking, in which vacuoles cluster and accumulate fusion proteins and fusion regulatory lipids at a ring-shaped microdomain surrounding the apposed membranes of each docked vacuole, where fusion will occur, and (iii) bilayer fusion/compartment mixing. These stages require vacuolar SNAREs, SNARE-chaperones, GTPases, effector complexes, and chemically minor but functionally important lipids. For each, we have developed specific ligands that block fusion and conditions that reverse each block. Using them, we test whether docking entails a linearly ordered series of catalytic events, marked by sequential acquisition of resistance to inhibitors, or whether docking subreactions are cooperative and/or reversible. We find that each fusion protein and regulatory lipid is needed throughout docking, indicative of a reversible or highly cooperative assembly of the fusion-competent vertex ring. In accord with this cooperativity, vertices enriched in one fusion catalyst are enriched in others. Docked vacuoles finally assemble SNARE complexes, yet still require physiological temperature and lipid rearrangements to complete fusion.

Original languageEnglish (US)
Pages (from-to)5260-5269
Number of pages10
JournalEMBO Journal
Volume25
Issue number22
DOIs
StatePublished - Nov 15 2006
Externally publishedYes

Fingerprint

Vacuoles
Yeast
Fusion reactions
SNARE Proteins
Yeasts
Lipids
GTP Phosphohydrolases
Cluster Analysis
Proteins
Ligands
Temperature
Membranes
Catalysts

Keywords

  • Membrane fusion
  • Organelle docking
  • Yeast vacuole

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Jun, Y., Thorngren, N., Starai, V. J., Fratti, R. A., Collins, K., & Wickner, W. (2006). Reversible, cooperative reactions of yeast vacuole docking. EMBO Journal, 25(22), 5260-5269. https://doi.org/10.1038/sj.emboj.7601413

Reversible, cooperative reactions of yeast vacuole docking. / Jun, Youngsoo; Thorngren, Naomi; Starai, Vincent J.; Fratti, Rutilio A.; Collins, Kevin; Wickner, William.

In: EMBO Journal, Vol. 25, No. 22, 15.11.2006, p. 5260-5269.

Research output: Contribution to journalArticle

Jun, Y, Thorngren, N, Starai, VJ, Fratti, RA, Collins, K & Wickner, W 2006, 'Reversible, cooperative reactions of yeast vacuole docking', EMBO Journal, vol. 25, no. 22, pp. 5260-5269. https://doi.org/10.1038/sj.emboj.7601413
Jun Y, Thorngren N, Starai VJ, Fratti RA, Collins K, Wickner W. Reversible, cooperative reactions of yeast vacuole docking. EMBO Journal. 2006 Nov 15;25(22):5260-5269. https://doi.org/10.1038/sj.emboj.7601413
Jun, Youngsoo ; Thorngren, Naomi ; Starai, Vincent J. ; Fratti, Rutilio A. ; Collins, Kevin ; Wickner, William. / Reversible, cooperative reactions of yeast vacuole docking. In: EMBO Journal. 2006 ; Vol. 25, No. 22. pp. 5260-5269.
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