Reversal of resistance to rhodamine 123 in adriamycin-resistant Friend leukemia cells

Theodore Lampidis, J. N. Munck, A. Krishan, H. Tapiero

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Abstract

Pleiotropic resistance to rhodamine 123 (Rho-123) in Adriamycin (ADM)-resistant Friend leukemia cells was circumvented by cotreatment with 10 μM verapamil. Increased cytotoxicity corresponded to higher intracellular Rho-123 levels. The verapamil-induced increase of drug accumulation in resistant cells is accounted for at least in part by the blockage or slowing of Rho-123 efflux from these cells. In contrast, accumulation and consequent cytotoxicity of Rho-123 in sensitive cells are not increased by verapamil. Similar results were obtained when ADM was used in this cell system. These results suggest that the efflux system for Rho-123 and ADM in sensitive cells is either reduced or absent. Although Rho-123 accumulates specifically in mitochondria and ADM mainly in the nucleus, the loss of these two different classes of compounds from resistant cells appears to occur via a similar or common mechanism. The similarities in drug transport between Rho-123 and ADM may have important implications when applied to an in vivo environment.

Original languageEnglish
Pages (from-to)2626-2631
Number of pages6
JournalCancer Research
Volume45
Issue number6
StatePublished - Jan 1 1985

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Rhodamine 123
Doxorubicin
Leukemia
Verapamil
Pharmaceutical Preparations
Mitochondria

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Reversal of resistance to rhodamine 123 in adriamycin-resistant Friend leukemia cells. / Lampidis, Theodore; Munck, J. N.; Krishan, A.; Tapiero, H.

In: Cancer Research, Vol. 45, No. 6, 01.01.1985, p. 2626-2631.

Research output: Contribution to journalArticle

Lampidis, T, Munck, JN, Krishan, A & Tapiero, H 1985, 'Reversal of resistance to rhodamine 123 in adriamycin-resistant Friend leukemia cells', Cancer Research, vol. 45, no. 6, pp. 2626-2631.
Lampidis, Theodore ; Munck, J. N. ; Krishan, A. ; Tapiero, H. / Reversal of resistance to rhodamine 123 in adriamycin-resistant Friend leukemia cells. In: Cancer Research. 1985 ; Vol. 45, No. 6. pp. 2626-2631.
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