Reversal of CD8+ T cell ignorance and induction of anti-tumor immunity by peptide-pulsed APC

N. Dalyot-Herman, O. F. Bathe, T. R. Malek

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

In the present report, we have studied the potential of naive and activated effector CD8+ T cells to function as anti-tumor T cells to a solid tumor using OVA-specific T cells from TCR-transgenic OT-I mice. Adoptive transfer of naive OT-I T cells into tumor-bearing syngeneic mice did not inhibit tumor cell growth. The adoptively transferred OT-I T cells did not proliferate in lymphoid tissue of tumor-bearing mice and were not anergized by the tumor. In contrast, adoptive transfer of preactivated OT-I CTL inhibited tumor growth in a dose-dependent manner, indicating that E.G7 was susceptible to immune effector cells. Importantly, naive OT-I T cells proliferated and elicited an anti-tumor response if they were adoptively transferred into normal or CD4-deficient mice that were then vaccinated with GM-CSF-induced bone marrow-derived OVA-pulsed APC. Collectively, these data indicate that even though naive tumor-specific T cells are present at a relatively high fraction they remain ignorant of the tumor and demonstrate that a CD8-mediated anti-tumor response can be induced by Ag-pulsed APC without CD4 T cell help.

Original languageEnglish (US)
Pages (from-to)6731-6737
Number of pages7
JournalJournal of Immunology
Volume165
Issue number12
DOIs
StatePublished - Dec 15 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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