Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer

J. A. Roth, Dao Nguyen, D. D. Lawrence, B. L. Kemp, C. H. Carrasco, D. Z. Ferson, W. K. Hong, R. Komaki, J. J. Lee, J. C. Nesbitt, K. M W Pisters, J. B. Putnam, R. Schea, D. M. Shin, G. L. Walsh, M. M. Dolormente, C. I. Han, F. D. Martin, N. Yen, K. XuL. C. Stephens, T. J. Mcdonnell, T. Mukhopadhyay, D. Cai

Research output: Contribution to journalArticle

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Abstract

A retroviral vector containing the wild-type p53 gene under control of a β-actin promoter was produced to mediate transfer of wild-type p53 into human non-small cell lung cancers by direct injection. Nine patients whose conventional treatments failed were entered into the study. No clinically significant vector-related toxic effects were noted up to five months after treatment. In situ hybridization and DNA polymerase chain reaction showed vector-p53 sequences in posttreatment biopsies. Apoptosis (programmed cell death) was more frequent in posttreatment biopsies than in pretreatment biopsies. Tumor regression was noted in three patients, and tumor growth stabilized in three other patients.

Original languageEnglish
Pages (from-to)985-991
Number of pages7
JournalNature Medicine
Volume2
Issue number9
DOIs
StatePublished - Sep 1 1996
Externally publishedYes

Fingerprint

Gene transfer
Biopsy
p53 Genes
Retroviridae
Tumors
Lung Neoplasms
Neoplasms
Poisons
Polymerase chain reaction
Direct injection
Cell death
DNA-Directed DNA Polymerase
Non-Small Cell Lung Carcinoma
In Situ Hybridization
Actins
Cell Death
Genes
Cells
Apoptosis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Roth, J. A., Nguyen, D., Lawrence, D. D., Kemp, B. L., Carrasco, C. H., Ferson, D. Z., ... Cai, D. (1996). Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer. Nature Medicine, 2(9), 985-991. https://doi.org/10.1038/nm0996-985

Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer. / Roth, J. A.; Nguyen, Dao; Lawrence, D. D.; Kemp, B. L.; Carrasco, C. H.; Ferson, D. Z.; Hong, W. K.; Komaki, R.; Lee, J. J.; Nesbitt, J. C.; Pisters, K. M W; Putnam, J. B.; Schea, R.; Shin, D. M.; Walsh, G. L.; Dolormente, M. M.; Han, C. I.; Martin, F. D.; Yen, N.; Xu, K.; Stephens, L. C.; Mcdonnell, T. J.; Mukhopadhyay, T.; Cai, D.

In: Nature Medicine, Vol. 2, No. 9, 01.09.1996, p. 985-991.

Research output: Contribution to journalArticle

Roth, JA, Nguyen, D, Lawrence, DD, Kemp, BL, Carrasco, CH, Ferson, DZ, Hong, WK, Komaki, R, Lee, JJ, Nesbitt, JC, Pisters, KMW, Putnam, JB, Schea, R, Shin, DM, Walsh, GL, Dolormente, MM, Han, CI, Martin, FD, Yen, N, Xu, K, Stephens, LC, Mcdonnell, TJ, Mukhopadhyay, T & Cai, D 1996, 'Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer', Nature Medicine, vol. 2, no. 9, pp. 985-991. https://doi.org/10.1038/nm0996-985
Roth, J. A. ; Nguyen, Dao ; Lawrence, D. D. ; Kemp, B. L. ; Carrasco, C. H. ; Ferson, D. Z. ; Hong, W. K. ; Komaki, R. ; Lee, J. J. ; Nesbitt, J. C. ; Pisters, K. M W ; Putnam, J. B. ; Schea, R. ; Shin, D. M. ; Walsh, G. L. ; Dolormente, M. M. ; Han, C. I. ; Martin, F. D. ; Yen, N. ; Xu, K. ; Stephens, L. C. ; Mcdonnell, T. J. ; Mukhopadhyay, T. ; Cai, D. / Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer. In: Nature Medicine. 1996 ; Vol. 2, No. 9. pp. 985-991.
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