Retrovirus D/New England and its relation to Mason-Pfizer monkey virus

Ronald Charles Desrosiers, M. D. Daniel, C. V. Butler, D. K. Schmidt, N. L. Letvin, R. D. Hunt, N. W. King, C. S. Barker, E. Hunter

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Seventeen isolates of retrovirus D/New England have been obtained from three species of macaques at the New England Regional Primate Research Center. Seven of the isolates were obtained from macaques who subsequently died with the macaque immunodeficiency syndrome; other isolates were obtained from macaques with less severe or other forms of illness. Attempts to isolate type D retrovirus from peripheral lymphocytes of 97 apparently healthy macaques have not been successful. Cloned DNA was prepared from Hirt supernatants of cells infected with one of these isolates (D/New England 398). By restriction endonuclease analysis, cloned pD398 DNA represented full-length viral DNA with one long terminal repeat. A detailed restriction endonuclease map of pD398 was derived and compared with a map of the cloned Mason-Pfizer monkey virus genome. Forty-six percent (13 of 28) of restriction endonuclease sites were found to be conserved when these related viruses were compared. Five of the D/New England isolates, including those from three different macaque species, were examined for strain variability by restriction endonuclease typing. Comparison of over 30 restriction endonuclease sites has not distinguished any of these D/New England isolates. It thus appears that a single strain of type D retrovirus is infecting three different species of macaques in the New England colony. Markedly reduced cross-hybridization was observed between cloned pD398 and Mason-Pfizer monkey virus DNAs at high stringency; this reduced cross-hybridization was localized to the pol-env regions of the genome. Only very weak hybridization of D/New England DNA to cloned squirrel monkey type D retrovirus DNA could be detected even at low-stringency conditions. What role type D retrovirus plays in the immunodeficiency syndrome of macaques remains to be determined.

Original languageEnglish (US)
Pages (from-to)552-560
Number of pages9
JournalJournal of Virology
Volume54
Issue number2
StatePublished - 1985
Externally publishedYes

Fingerprint

Mason-Pfizer monkey virus
Retroviridae
New England
New England region
Macaca
Betaretrovirus
DNA Restriction Enzymes
restriction endonucleases
DNA
hybridization
immunosuppression
Simian Retroviruses
Genome
Saimiri
terminal repeat sequences
restriction mapping
genome
Terminal Repeat Sequences
Viral DNA
Primates

ASJC Scopus subject areas

  • Immunology

Cite this

Desrosiers, R. C., Daniel, M. D., Butler, C. V., Schmidt, D. K., Letvin, N. L., Hunt, R. D., ... Hunter, E. (1985). Retrovirus D/New England and its relation to Mason-Pfizer monkey virus. Journal of Virology, 54(2), 552-560.

Retrovirus D/New England and its relation to Mason-Pfizer monkey virus. / Desrosiers, Ronald Charles; Daniel, M. D.; Butler, C. V.; Schmidt, D. K.; Letvin, N. L.; Hunt, R. D.; King, N. W.; Barker, C. S.; Hunter, E.

In: Journal of Virology, Vol. 54, No. 2, 1985, p. 552-560.

Research output: Contribution to journalArticle

Desrosiers, RC, Daniel, MD, Butler, CV, Schmidt, DK, Letvin, NL, Hunt, RD, King, NW, Barker, CS & Hunter, E 1985, 'Retrovirus D/New England and its relation to Mason-Pfizer monkey virus', Journal of Virology, vol. 54, no. 2, pp. 552-560.
Desrosiers RC, Daniel MD, Butler CV, Schmidt DK, Letvin NL, Hunt RD et al. Retrovirus D/New England and its relation to Mason-Pfizer monkey virus. Journal of Virology. 1985;54(2):552-560.
Desrosiers, Ronald Charles ; Daniel, M. D. ; Butler, C. V. ; Schmidt, D. K. ; Letvin, N. L. ; Hunt, R. D. ; King, N. W. ; Barker, C. S. ; Hunter, E. / Retrovirus D/New England and its relation to Mason-Pfizer monkey virus. In: Journal of Virology. 1985 ; Vol. 54, No. 2. pp. 552-560.
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