Retroviral vector-mediated γ-interferon gene transfer into tumor cells generates potent and long lasting antitumor immunity

Bernd Gansbacher, Rajat Bannerji, Brian Daniels, Karen Zier, Eli Gilboa, Eli Gilboa

Research output: Contribution to journalArticle

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Abstract

Retroviral vectors were used to introduce the γ-interferon (IFN-γ) gene into CMS-5 cells, a weakly immunogenic tumor of BALB/c origin. After selection in G418-containing medium, colonies were isolated, cloned, and expanded to cell lines. IFN-γ secretion was assessed using a bioassay and enzyme-linked immunosorbent assay, and high (25 units/ ml) and low (5 units/ml) IFN-γ producers were isolated. Tumor growth was followed after intradermal injection, and spleen cells were isolated at different time points. IFN-γ secretion by tumor cells abrogated their tumorigenicity and induced a persistent and specific antitumor immunity. In contrast to the normally observed cellular immunosuppression in unmodified CMS-5 tumor-bearing mice, IFN-γ-producing tumors induced a long lasting state of T-cell immunity, as judged by rejection of a CMS-5 tumor challenge and persistence of specific cytotoxic activity in the spleen cell population. High levels of tumor-specific cytotoxic activity could also be detected if IFN-γ-secreting tumor cells, but not unmodified CMS-5 cells, were used as targets at a time point when immunosuppression was usually seen. These studies highlight the potential advantages of localized IFN-γ secretion to induce potent antitumor immune responses.

Original languageEnglish
Pages (from-to)7820-7825
Number of pages6
JournalCancer Research
Volume50
Issue number24
StatePublished - Dec 15 1990
Externally publishedYes

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Interferons
Immunity
Genes
Neoplasms
Immunosuppression
Spleen
Intradermal Injections
Biological Assay
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Cell Line
Growth
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Retroviral vector-mediated γ-interferon gene transfer into tumor cells generates potent and long lasting antitumor immunity. / Gansbacher, Bernd; Bannerji, Rajat; Daniels, Brian; Zier, Karen; Gilboa, Eli; Gilboa, Eli.

In: Cancer Research, Vol. 50, No. 24, 15.12.1990, p. 7820-7825.

Research output: Contribution to journalArticle

Gansbacher, B, Bannerji, R, Daniels, B, Zier, K, Gilboa, E & Gilboa, E 1990, 'Retroviral vector-mediated γ-interferon gene transfer into tumor cells generates potent and long lasting antitumor immunity', Cancer Research, vol. 50, no. 24, pp. 7820-7825.
Gansbacher, Bernd ; Bannerji, Rajat ; Daniels, Brian ; Zier, Karen ; Gilboa, Eli ; Gilboa, Eli. / Retroviral vector-mediated γ-interferon gene transfer into tumor cells generates potent and long lasting antitumor immunity. In: Cancer Research. 1990 ; Vol. 50, No. 24. pp. 7820-7825.
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