Retroviral replicating vectors in cancer

Christopher R. Logg, Joan M. Robbins, Douglas J. Jolly, Harry E. Gruber, Noriyuki Kasahara

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

The use of replication-competent viruses for the treatment of cancer is an emerging technology that shows significant promise. Among the various different types of viruses currently being developed as oncolytic agents, retroviral replicating vectors (RRVs) possess unique characteristics that allow highly efficient, non-lytic, and tumor-selective gene transfer. By retaining all of the elements necessary for viral replication, RRVs are capable of transmitting genes via exponential in situ amplification. Their replication-competence also provides a powerful means whereby novel and useful RRV variants can be generated using natural selection. Their stringent requirement for cell division in order to achieve productive infection, and their preferential replication in cells with defective innate immunity, confer a considerable degree of natural specificity for tumors. Furthermore, their ability to integrate stably into the genome of cancer cells, without immediate cytolysis, contributes to long-lasting therapeutic efficacy. Thus, RRVs show much promise as therapeutic agents for cancer and are currently being tested in the clinic. Here we describe experimental methods for their production and quantitation, for adaptive evolution and natural selection to develop novel or improved RRV, and for in vitro and in vivo assessment of the therapeutic efficacy of RRVs carrying prodrug activator genes for treatment of cancer.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages199-228
Number of pages30
DOIs
StatePublished - 2012

Publication series

NameMethods in Enzymology
Volume507
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Glioblastoma
  • Oncolytic virotherapy
  • Prodrug activator gene therapy
  • Replicating virus vector
  • Replication-competent retrovirus
  • Suicide gene therapy

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Retroviral replicating vectors in cancer'. Together they form a unique fingerprint.

  • Cite this

    Logg, C. R., Robbins, J. M., Jolly, D. J., Gruber, H. E., & Kasahara, N. (2012). Retroviral replicating vectors in cancer. In Methods in Enzymology (pp. 199-228). (Methods in Enzymology; Vol. 507). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-386509-0.00011-9