Retinoic acid combined with neurotrophin-3 enhances the survival and neurite outgrowth of embryonic sympathetic neurons

Lori A. Plum, Luis F. Parada, Pantelis Tsoulfas, Margaret Clagett-Dame

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Both nerve growth factor (NGF) and neurotrophin-3 (NT-3) are necessary for the survival of embryonic sympathetic neurons in vivo. All-trans retinoic acid (atRA) has been shown to promote neurite outgrowth and long-term survival of chick embryonic sympathetic neurons cultured in the presence of NGF. The present study shows that atRA can also potentiate the survival and neurite outgrowth-promoting activities of NT-3. This was accomplished by enhancing the survival of existing neurons, as cell proliferation was unaffected by exposure to atRA. atRA also enhanced neurite outgrowth of the NT-3-treated cells; however, the neurites appeared thicker and less branched than cells treated with atRA in combination with NGF. Using a quantitative PCR assay, trkA and p75NTR mRNAs, but not trkC mRNA, were increased (∼1.5- to 2-fold) after 72 and 48 hr of exposure of the cultures to atRA, respectively. The atRA-induced increase in trkA mRNA may play a role in the enhanced survival of neurons cultured in the presence of either NGF or NT-3, as both neurotrophins have been shown to signal through this receptor. The time course of these mRNA changes would indicate that atRA does not regulate the neurotrophin receptor mRNA directly, rather, intervening gene transcription is required. Thus, during development, atRA may play a role in fine-tuning embryonic responsiveness to both NT-3 and NGF.

Original languageEnglish
Pages (from-to)766-775
Number of pages10
JournalExperimental Biology and Medicine
Volume226
Issue number8
StatePublished - Sep 6 2001

Fingerprint

Neurotrophin 3
Tretinoin
Neurons
Nerve Growth Factor
Messenger RNA
Nerve Growth Factor Receptors
Neuronal Outgrowth
Nerve Growth Factors
Cell proliferation
Neurites
Transcription
Assays
Tuning
Genes
Cells
Cell Proliferation
Polymerase Chain Reaction

Keywords

  • Nerve growth factor
  • Neurotrophin-3
  • p75
  • Retinoic acid
  • Sympathetic neuron
  • TrkA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Retinoic acid combined with neurotrophin-3 enhances the survival and neurite outgrowth of embryonic sympathetic neurons. / Plum, Lori A.; Parada, Luis F.; Tsoulfas, Pantelis; Clagett-Dame, Margaret.

In: Experimental Biology and Medicine, Vol. 226, No. 8, 06.09.2001, p. 766-775.

Research output: Contribution to journalArticle

@article{233f50ce713e477d995677e3eb5591da,
title = "Retinoic acid combined with neurotrophin-3 enhances the survival and neurite outgrowth of embryonic sympathetic neurons",
abstract = "Both nerve growth factor (NGF) and neurotrophin-3 (NT-3) are necessary for the survival of embryonic sympathetic neurons in vivo. All-trans retinoic acid (atRA) has been shown to promote neurite outgrowth and long-term survival of chick embryonic sympathetic neurons cultured in the presence of NGF. The present study shows that atRA can also potentiate the survival and neurite outgrowth-promoting activities of NT-3. This was accomplished by enhancing the survival of existing neurons, as cell proliferation was unaffected by exposure to atRA. atRA also enhanced neurite outgrowth of the NT-3-treated cells; however, the neurites appeared thicker and less branched than cells treated with atRA in combination with NGF. Using a quantitative PCR assay, trkA and p75NTR mRNAs, but not trkC mRNA, were increased (∼1.5- to 2-fold) after 72 and 48 hr of exposure of the cultures to atRA, respectively. The atRA-induced increase in trkA mRNA may play a role in the enhanced survival of neurons cultured in the presence of either NGF or NT-3, as both neurotrophins have been shown to signal through this receptor. The time course of these mRNA changes would indicate that atRA does not regulate the neurotrophin receptor mRNA directly, rather, intervening gene transcription is required. Thus, during development, atRA may play a role in fine-tuning embryonic responsiveness to both NT-3 and NGF.",
keywords = "Nerve growth factor, Neurotrophin-3, p75, Retinoic acid, Sympathetic neuron, TrkA",
author = "Plum, {Lori A.} and Parada, {Luis F.} and Pantelis Tsoulfas and Margaret Clagett-Dame",
year = "2001",
month = "9",
day = "6",
language = "English",
volume = "226",
pages = "766--775",
journal = "Experimental Biology and Medicine",
issn = "0037-9727",
publisher = "Society for Experimental Biology and Medicine",
number = "8",

}

TY - JOUR

T1 - Retinoic acid combined with neurotrophin-3 enhances the survival and neurite outgrowth of embryonic sympathetic neurons

AU - Plum, Lori A.

AU - Parada, Luis F.

AU - Tsoulfas, Pantelis

AU - Clagett-Dame, Margaret

PY - 2001/9/6

Y1 - 2001/9/6

N2 - Both nerve growth factor (NGF) and neurotrophin-3 (NT-3) are necessary for the survival of embryonic sympathetic neurons in vivo. All-trans retinoic acid (atRA) has been shown to promote neurite outgrowth and long-term survival of chick embryonic sympathetic neurons cultured in the presence of NGF. The present study shows that atRA can also potentiate the survival and neurite outgrowth-promoting activities of NT-3. This was accomplished by enhancing the survival of existing neurons, as cell proliferation was unaffected by exposure to atRA. atRA also enhanced neurite outgrowth of the NT-3-treated cells; however, the neurites appeared thicker and less branched than cells treated with atRA in combination with NGF. Using a quantitative PCR assay, trkA and p75NTR mRNAs, but not trkC mRNA, were increased (∼1.5- to 2-fold) after 72 and 48 hr of exposure of the cultures to atRA, respectively. The atRA-induced increase in trkA mRNA may play a role in the enhanced survival of neurons cultured in the presence of either NGF or NT-3, as both neurotrophins have been shown to signal through this receptor. The time course of these mRNA changes would indicate that atRA does not regulate the neurotrophin receptor mRNA directly, rather, intervening gene transcription is required. Thus, during development, atRA may play a role in fine-tuning embryonic responsiveness to both NT-3 and NGF.

AB - Both nerve growth factor (NGF) and neurotrophin-3 (NT-3) are necessary for the survival of embryonic sympathetic neurons in vivo. All-trans retinoic acid (atRA) has been shown to promote neurite outgrowth and long-term survival of chick embryonic sympathetic neurons cultured in the presence of NGF. The present study shows that atRA can also potentiate the survival and neurite outgrowth-promoting activities of NT-3. This was accomplished by enhancing the survival of existing neurons, as cell proliferation was unaffected by exposure to atRA. atRA also enhanced neurite outgrowth of the NT-3-treated cells; however, the neurites appeared thicker and less branched than cells treated with atRA in combination with NGF. Using a quantitative PCR assay, trkA and p75NTR mRNAs, but not trkC mRNA, were increased (∼1.5- to 2-fold) after 72 and 48 hr of exposure of the cultures to atRA, respectively. The atRA-induced increase in trkA mRNA may play a role in the enhanced survival of neurons cultured in the presence of either NGF or NT-3, as both neurotrophins have been shown to signal through this receptor. The time course of these mRNA changes would indicate that atRA does not regulate the neurotrophin receptor mRNA directly, rather, intervening gene transcription is required. Thus, during development, atRA may play a role in fine-tuning embryonic responsiveness to both NT-3 and NGF.

KW - Nerve growth factor

KW - Neurotrophin-3

KW - p75

KW - Retinoic acid

KW - Sympathetic neuron

KW - TrkA

UR - http://www.scopus.com/inward/record.url?scp=0034859732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034859732&partnerID=8YFLogxK

M3 - Article

C2 - 11520943

AN - SCOPUS:0034859732

VL - 226

SP - 766

EP - 775

JO - Experimental Biology and Medicine

JF - Experimental Biology and Medicine

SN - 0037-9727

IS - 8

ER -