Response element sequence modulates estrogen receptor α and β affinity and activity

P. C. Kulakosky, M. A. McCarty, S. C. Jernigan, K. E. Risinger, C. M. Klinge

Research output: Contribution to journalReview article

42 Scopus citations

Abstract

The relationship between estrogen receptor (ER)-estrogen response element (ERE) binding affinity and estradiol (E2)-induced transcription has not been systematically or quantitatively tested. We examined the influence of ERE palindrome length and the 3′ ERE flanking sequence on ERα and ERβ affinity binding in vitro and on the induction of reporter gene activity in transfected cells. The addition of one nucleotide in each arm of the 13 bp ERE palindrome, forming a 15 bp ERE palindrome, increased ERα and ERβ affinity and transcription. In contrast, the addition of an AT-rich flanking sequence from genes highly stimulated by E2 had little effect on affinity or reporter gene activity. Notable differences between ERα and ERβ include: both Kd and transcriptional induction were generally higher for ERα than ERβ, better correlation between ERE palindrome length and transcriptional induction for ERα than ERβ, and a better correlation between (ER-ERE) Kd and transcriptional induction for ERα than for ERβ.

Original languageEnglish (US)
Pages (from-to)137-152
Number of pages16
JournalJournal of Molecular Endocrinology
Volume29
Issue number1
DOIs
StatePublished - Aug 1 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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    Kulakosky, P. C., McCarty, M. A., Jernigan, S. C., Risinger, K. E., & Klinge, C. M. (2002). Response element sequence modulates estrogen receptor α and β affinity and activity. Journal of Molecular Endocrinology, 29(1), 137-152. https://doi.org/10.1677/jme.0.0290137