Respiratory supercomplexes enhance electron transport by decreasing cytochrome c diffusion distance

Jens Berndtsson, Andreas Aufschnaiter, Sorbhi Rathore, Lorena Marin-Buera, Hannah Dawitz, Jutta Diessl, Verena Kohler, Antoni Barrientos, Sabrina Büttner, Flavia Fontanesi, Martin Ott

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Respiratory chains are crucial for cellular energy conversion and consist of multi-subunit complexes that can assemble into supercomplexes. These structures have been intensively characterized in various organisms, but their physiological roles remain unclear. Here, we elucidate their function by leveraging a high-resolution structural model of yeast respiratory supercomplexes that allowed us to inhibit supercomplex formation by mutation of key residues in the interaction interface. Analyses of a mutant defective in supercomplex formation, which still contains fully functional individual complexes, show that the lack of supercomplex assembly delays the diffusion of cytochrome c between the separated complexes, thus reducing electron transfer efficiency. Consequently, competitive cellular fitness is severely reduced in the absence of supercomplex formation and can be restored by overexpression of cytochrome c. In sum, our results establish how respiratory supercomplexes increase the efficiency of cellular energy conversion, thereby providing an evolutionary advantage for aerobic organisms.

Original languageEnglish (US)
Article numbere51015
JournalEMBO Reports
Volume21
Issue number12
DOIs
StatePublished - Dec 3 2020

Keywords

  • bioenergetics
  • competitive fitness
  • cryo-EM
  • mitochondria
  • respiratory chain supercomplexes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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